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Protection against lethal Sendai virus infection by in vivo priming of virus-specific cytotoxic T lymphocytes with free synthetic peptide

Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 04/1991; 88(6):2283-7. DOI: 10.1073/pnas.88.6.2283
Source: PubMed

ABSTRACT The only peptide of Sendai virus that is recognized by cytotoxic T lymphocytes (CTL) in B6 mice was found with (i) the use of recombinant vaccinia virus constructs containing separate genes of Sendai virus and (ii) a set of overlapping peptides completely spanning the identified nucleoprotein (NP) gene product. This immunodominant NP peptide is recognized by Sendai virus-specific CTL that are known to have therapeutic effects in vivo. By subcutaneous immunization, this peptide induced Sendai virus and NP peptide-specific CTL memory responses in vivo. Most importantly, mice that had been immunized with this peptide were protected against a lethal virus dose, indicating that viral peptides can be used as antiviral T-cell vaccines. The induction of T-cell memory by free peptide immunization potentially has wide applicability in biology and medicine, including protection against infectious disease.

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Available from: Wijbe Martin Kast, Aug 20, 2015
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