The nerve growth factor receptor gene is expressed in both neuronal and non-neuronal tissues in the human fetus
ABSTRACT In situ hybridization was used to study expression of beta-nerve growth factor receptor (NGF-R) mRNA in the early human fetus. In 8- to 12-week old fetuses, high labelling was found over motoneurons along the entire length of the lateral motor column. High levels of NGF-R mRNA were also seen over most developing nerve cell bodies in both the dorsomedial and ventrolateral part of the dorsal root ganglia. Lower, but clearly specific labelling was detected over a subpopulation of cells in Auerbach's plexus in the intestines. Evidence for a non-neuronal expression of NGF-R mRNA came from labelling over a subpopulation of cells in glomeruli of the kidney in a 12-week old human embryo. Myoblasts in skeletal muscle anlagen were labelled as well as cells along peripheral nerve. The widespread expression of NGF-R mRNA in the human fetus suggests that the NGF-R is important for development of a variety of different tissues of both neuronal and non-neuronal origin.
- SourceAvailable from: Silvia Brunelli
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- "The expression of NGF has been studied both in human and animal models of myogenesis: NGF is expressed in developing muscle (Ernfors et al., 1991; Wheeler and Bothwell, 1992; Ip et al., 2001) and is down-regulated after birth (Ernfors et al., 1991; Ip et al., 2001). It appears to be reexpressed in adult muscle in the course of pathological conditions such as muscular dystrophy (Toti et al., 2003) and amyotrophic lateral sclerosis (Kust et al., 2002): de novo expression of NGF occurs in regenerating fibers and connective tissue myofibroblasts of the damaged muscles (Toti et al., 2003; Chevrel et al., 2006), suggesting that it may have a proregenerative role (Menetrey et al., 2000). "
ABSTRACT: Regeneration of muscle fibers, lost during pathological muscle degeneration or after injuries, is mediated by the production of new myofibres. This process, sustained by the resident stem cells of the muscle, the satellite cells, is finely regulated by local cues, in particular by cytokines and growth factors. Evidence in the literature suggests that nerve growth factor (NGF) is involved in muscle fiber regeneration; however, its role and mechanism of action were unclear. We have investigated this issue in in vivo mouse models of muscle regeneration and in primary myogenic cells. Our results demonstrate that NGF acts through its low-affinity receptor p75(NTR) in a developmentally regulated signaling pathway necessary to myogenic differentiation and muscle repair in vivo. We also demonstrate that this action of NGF is mediated by the down-regulation of RhoA-GTP signaling in myogenic cells.Molecular biology of the cell 07/2009; 20(16):3620-7. DOI:10.1091/mbc.E09-01-0012 · 4.47 Impact Factor
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- "In addition, in situ hybridization has revealed NGFR mRNA in rat teeth (Byers et al., 1990). However, limited information is available regarding NGFR expression during prenatal development in humans (Ernfors et al., 1991; Christensen et al., 1993). To date, there are no studies regarding NGFR immunoreactivity in the palate and tongue in human fetuses. "
ABSTRACT: To investigate the temporospatial pattern of nerve growth factor receptor (NGFR) immunolocalization during human palatal closure. Human palate and tongue tissues from 33 embryos/fetuses, 9 to 22 weeks of fertilization age. Tissues were divided according to developmental stage and palatal development (before, during, and after closure) and then subjected to decalcification, paraffin embedding, serial sectioning, survey staining, and p75NGFR immunohistochemical staining. Specific temporospatial patterns of p75NGFR reactivity were observed; reactivity was intense in the soft tissue palatal shelves before and during palatal closure and was weaker in the palate after palatal closure. In the tongue, intense reactivity was seen throughout 9 to 22 weeks. The observed patterns suggest that p75NGFR may enable the visualization of physiological events in palatal closure during normal human development.The Cleft Palate-Craniofacial Journal 04/2003; 40(2):116-25. DOI:10.1597/1545-1569(2003)040<0116:NGFRID>2.0.CO;2 · 1.20 Impact Factor
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ABSTRACT: Several projects that would be used for nuclear physics research are at various stages of development or construction. The fixed target projects include a hadron facility now under construction at JAERI and an upgrade of the CEBAF 6 GeV electron facility at Jefferson Lab to 12 GeV. eRHIC is the proposed electron-ion collider at Brookhaven National Laboratory. Jefferson Lab also proposed a project that combines a 25 GeV fixed target capability with an electron-ion collider, eLIC. The conceptual layouts for all the projects are presented as is the utilization of srf technology. The R&D opportunities are discussed.