Determination of phenol, m-, o- and p-cresol, p-aminophenol and p-nitrophenol in urine by high-performance liquid chromatography

Laboratorio Biomed, (Concesio) BS, Italy.
Journal of Chromatography A (Impact Factor: 4.17). 01/1991; 535(1-2):311-6. DOI: 10.1016/S0021-9673(01)88957-3
Source: PubMed


A method for the biological monitoring of human exposure to aromatic hydrocarbons, nitrocompounds, amines and phenols has been developed. Phenol, cresols, p-aminophenol, p-nitrophenol and their glucorono- or sulpho-conjugates, were quantified by HPLC; 4-chlorphenol was added as internal standard. After enzymatic hydrolysis, the free compounds were extracted with an organic solvent and analyzed by an isocratic HPLC Perkin Elmer system at ambient temperature and at a flow-rate of 1 ml/min. The column was a reversed-phase Pecosphere 3 x 3 C18 Perkin Elmer; the mobile phase was a 30:70:0.1 (v/v/v) methanol-water-orthophosphoric acid mixture and the chromatogram was monitored at 215 nm. Identification was based on retention time and quantification was performed by automatic peak height determination, corrected for the internal standard. The recovery was ca. 95% for phenol and cresols; 90% for p-nitrophenol; 85% for p-aminophenol; the coefficients of variance were less than 6% within analysis (n = 20) and less than 10% between analysis (n = 20). The detection limits, at a signal/noise ratio of 2, were 0.5 mg/l for phenol and cresols and 1 mg/l for p-aminophenol and p-nitrophenol.

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    • "It is focused a significant attention for the development of simple, reliable, and ultrasensitive in various detection methodology based on undoped nanomaterials. Generally, toxic 4-NPh determination is performed using conventional chromatographic techniques, such as gas-chromatography (GC) [47] [48], high-performance liquid chromatography (HPLC) [49] [50], liquid chromatography (LC) connected with mass-spectroscopy. Universal screening method for the determination of US Environmental Protection Agency, phenols at nanolevel in water samples by on-line solid-phase extraction–highperformance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry within a single-run [51] and capillary-electrophoresis [52]. "
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    ABSTRACT: We have prepared low-dimensional silver oxide nanoparticles (NPs) by a sono-chemical methodusing reducing agents in alkaline medium. The resulting NPs were characterized by UV/vis and FT-IRspectroscopy, X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), X-ray energydispersive spectrometry (XEDS), and field-emission scanning electron microscopy (FESEM). They weredeposited on a flat-polycrystalline gold electrode (AuE, surface area, 0.0216 cm2) to give a sensor witha fast response toward 4-nitrophenol (4-NPh) in liquid phase. The sensor also displays good sensitiv-ity and long-term stability, and enhanced electrochemical performances. The calibration plot is linear(r2= 0.9873) over the large concentration range (LDR, 1.0 �M to 0.5 mM). The sensitivity and detectionlimit is calculated to ∼4.740 �A cm−2mM−1and ∼0.19 �M (signal-to-noise ratio, at a SNR of 3), respec-tively. We also discuss possible future prospective uses of this metal oxide nanomaterials in terms ofchemical sensing.
    Electrochimica Acta 12/2013; 112:422– 430. DOI:10.1016/j.electacta.2013.08.164 · 4.50 Impact Factor
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    • "There is focused a significant attention for the development of simple, reliable, and ultra-sensitive in various detection methodology based on codoped nanomaterials. Generally, the detection of toxic 4-nitrophenol is consummated using chromatographic techniques, such as gas-chromatography [27,28], high-performance liquid chromatography [29,30], liquid chromatography connected with mass-spectroscopy [31], and capillary-electrophoresis [32]. Electrochemical technique, which can offer fast, reliable, and direct real-time monitoring is one of the most utilized methods in the determination of nitro-phenolic stuffs. "
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    Chemistry Central Journal 03/2013; 7(1):60. DOI:10.1186/1752-153X-7-60 · 2.19 Impact Factor
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