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Karyotyping in plants by an image analysis system.

J.Genetics & Breeding 01/1991; 45.
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    ABSTRACT: A program system for microcomputer assisted karyotype analysis of banded chromosomes is presented. Measurement is done with a digitizer pad (graphics tablett), the quantification and statistical calculation is processed by a computer program with a microcomputer DEC PDP11/23. The significant karyotype parameters can be calculated and plotted in an idiogram: length of the chromosome arms, centromeric index, position of secondary constriction, position and size of bands. The program system is exemplified in C-banded Hyacinthoides italica s.l. (Hyacinthaceae), which shows a rather complex banding pattern.
    Caryologia -Firenze- 01/2014; 50(2):105-116. DOI:10.1080/00087114.1997.10797390 · 0.85 Impact Factor
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    ABSTRACT: Karyotypic studies were carried out on 16 hull-less barley (Hordeum vulgare L.) genotypes, using squash technique and Aceto-orcein staining method. Chromosomal parameters examined were as follows: long arm (L), short arm (S), total chromosome length (TL), arm ratio (AR), r-value (S/L), form percentage of chromosome (%F), Chromosome volume, relative length of chromosome (%RL) and the number of satellites. ANOVA i indicated high significant differences for all karyotypic parameters. Genotypes tested were diploid (2n=2x=14). Satellite numbers were differed, ranging from 1 to 2 pairs and were differed in satellite length. The most chromatin length were detected in G9 (73.37 µm) while G15 demonstrated the least (30.85 µm). The types of chromosomes were determined as m in all genotypes, using Levan's chromosome nomenclature. Karyotypes were classified in 1A of Stebbin's classification. In addition to this, to test the karyotypic symmetry in more detail, other parameters, e.g. Romero-Zarco, total form percentage of karyotype (%TF), symmetry index (%S), coefficient of variation (%CV), dispersion index (DI) were also considered. For instance, in Romero-Zarco method, the A1 and A2 coefficients were 0.37 (G2) and 0.46 (G9), respectively. The first 3 principal component analysis PCA justified %94 of the total variations correlation determined for cytogenetical parameters. Cluster analysis was carried out for either chromosomal parameters classifying in 3 classes.
    Turkish Journal of Field Crops 11/2011; 16:245-250. · 0.64 Impact Factor

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