Conference Paper

Study the Biological Activities of Tribulus Terrestris Extracts

Conference: World Academy of Science, Engineering and Technology 57 2009


In this study the extracts of the Iraqi herb Tribulus
terrestris (Al-Hassage or Al-Kutub) was done by using of polar and
non polar solvents, then the biological activity of these extractants
was studied in three fields, First, the antibacterial activity (in vitro)
on gram positive bacteria (Staphylococcus aureus), and gram
negative bacteria (E. coli, Proteus vulgaris, Pseudomonas
aerugiuosa, and Klebsiella), all extracts showed considerable activity
against all bacteria. Second, the effect of extracts on free serum
testosterone level in male mice (in vivo), the alcoholic, and
acetonitrilic extracts showed significant (P < 0.05) increase in free
serum testosterone level, and we found that the extracts contained
compounds with less genotoxic effects in mice germ cells. 3rd, was to
study the effect of methanolic extract of T. terrestris in diabetes

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    • "Studies from other laboratories have previously indicated the endocrine disrupting potential of T. terrestris, particularly, the water, methanol, and ethanol extracts of T. terrestris. Hussain et al. (2009) showed that methanol extracts of T. terrestris increased free serum testosterone levels in male mice. Gauthaman et al. (2003) reported that the pro-erectile, aphrodisiac property of the water extracts of T. terrestris might result from an increase in androgen production and a subsequent release of nitric oxide from the nerve endings innervating the corpus cavernosum. "
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    ABSTRACT: Abstract Context: Tribulus terrestris L. (Zygophyllaceae) has been commonly used to energize, vitalize, and improve sexual function and physical performance in men. Objective: This study investigates the potential cytotoxic and genotoxic, and endocrine disrupting activities of T. terrestris in vitro. Materials and methods: The whole T. terrestris plant was extracted with water, methanol, and chloroform. The genotoxic potential of T. terrestris extracts at 3-2400 µg/mL was assessed by Comet assay in a rat kidney cell line (NRK-52E) and by Ames assay in Salmonella typhimurium TA98 and TA100 strains. Endocrine disrupting effects of the extracts at concentrations of 0.22-25 000 µg/mL were assessed by YES/YAS assay in Saccharomyces cerevisiae. Cytotoxic activity of the extracts was determined by the MTT test in NRK-52E cells. The different exposure times were used for four tests (3-48 h). Results: The methanol extract of T. terrestris IC50 value was 160 µg/mL. The other extracts did not show cytotoxic effects. In the Comet and Ames genotoxicity assays, none of the extracts possessed genotoxic activities at concentrations of 0-2400 µg/mL. Only the water extract of T. terrestris induced frame shift mutations after metabolic activation. The water extract also showed estrogenic activity by YES/YAS assay in S. cerevisiae at concentrations ≥27 µg/mL (≥2.6-fold), while the other T. terrestris extracts had anti-estrogenic properties. Conclusion: Tribulus terrestris had estrogenic and genotoxic activities. The study was useful in determining its toxicological effects and the precautions regarding consumption.
    Pharmaceutical Biology 12/2014; 53(4):1-8. DOI:10.3109/13880209.2014.924019 · 1.24 Impact Factor
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    • "There are reports showing that this extract decreases blood cholesterol levels in humans, rats and mice. However, data concerning the effect of T. terrestris extract on poultry are not present.[6] One of the uses of T. terrestris is in urinary infections. "
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    ABSTRACT: 2014): In vitro screening for anti-acetylcholiesterase, anti-oxidant, anti-glucosidase, anti-inflammatory and anti-bacterial effect of three traditional medicinal plants, Biotechnology & Biotechnological Equipment, makes every effort to ensure the accuracy of all the information (the "Content") contained in the publications on our platform. Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Versions of published Taylor & Francis and Routledge Open articles and Taylor & Francis and Routledge Open Select articles posted to institutional or subject repositories or any other third-party website are without warranty from Taylor & Francis of any kind, either expressed or implied, including, but not limited to, warranties of merchantability, fitness for a particular purpose, or non-infringement. Any opinions and views expressed in this article are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor & Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content.
    Biotechnology & Biotechnological Equipment 10/2014; DOI:10.1080/13102818.2014.969877 · 0.30 Impact Factor
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    • "It is used in folk medicine as tonic, aphrodisiac, analgesic, astringent, stomachic, anti-hypertensive, diuretic, lithontriptic and urinary anti-infective (Ody 2000). Acetone extracts of the leaves and seeds from T. terrestris showed moderate activity against the third-instar larvae of Anopheles culicifacies , A. stephensi, C. quinquefasciatus and Aedes aegypti (Singh et al. 2008), and the alcoholic, methanolic, acetonitrilic , and hexanic extracts of T. terrestris showed effective in vitro antibacterial activity against Staphylococcus aureus , Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa and Klebsiella (Hussain et al. 2009). "
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    ABSTRACT: The emergence and spread of Plasmodium falciparum with resistance to chloroquine (CQ), the safest and cheapest anti-malarial drug, coupled with the increasing cost of alternative drugs especially in developing countries have necessitated the urgent need to tap the potential of plants for novel anti-malarials. The present study investigates the anti-malarial activity of the methanolic extracts of 13 medicinal plants from the Malaiyur and Javadhu hills of South India against blood stage CQ-sensitive (3D7) and CQ-resistant (INDO) strains of P. falciparum in culture using the fluorescence-based SYBR Green I assay. Sorbitol-synchronized parasites were incubated under normal culture conditions at 2% hematocrit and 1% parasitemia in the absence or presence of increasing concentrations of plant extracts. CQ and artemisinin were used as positive controls, while 0.4% DMSO was used as the negative control. The cytotoxic effects of extracts on host cells were assessed by functional assay using HeLa cells cultured in RPMI containing 10% fetal bovine serum, 0.21% sodium bicarbonate and 50 μg/mL gentamycin (complete medium). Plant extracts (bark methanol extracts of Annona squamosa (IC(50), 30 μg/mL), leaf extracts of Ocimum gratissimum (IC(50), 32 μg/mL), Ocimum tenuiflorum (IC(50), 31 μg/mL), Solanum torvum (IC(50), 31 μg/mL) and Justicia procumbens (IC(50), 63 μg/mL), showed moderate activity. The leaf extracts of Aristolochia indica (IC(50), 10 μg/mL), Cassia auriculata (IC(50), 14 μg/mL), Chrysanthemum indicum (IC(50), 20 μg/mL) and Dolichos biflorus (IC(50), 20 μg/mL) showed promising activity and low activity was observed in the flower methanol extracts of A. indica , leaf methanol extract of Catharanthus roseus, and Gymnema sylvestre (IC(50), >100 μg/mL). These four extracts exhibited promising IC(50) (μg/mL) of 17, 24, 19 and 24 respectively also against the CQ resistant INDO strain of P. falciparum. The high TC(50) in mammalian cell cytotoxicity assay and the low IC(50) in anti-malarial P. falciparum assay indicates selectivity and good resistance indices in the range of 0.9-1.7 for leaf extracts of A. indica, C. auriculata, C. indicum and D. biflorus suggests that these may serve as anti-malarial agents even in their crude form. These results indicate a possible explanation of the traditional use of some of these medicinal plants against malaria or malaria-like conditions.
    Parasitology Research 06/2011; 111(2):703-15. DOI:10.1007/s00436-011-2457-6 · 2.10 Impact Factor
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