Article

Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure. Early phase (30 minutes).

Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550.
The American review of respiratory disease (impact factor: 10.19). 01/1991; 142(6 Pt 1):1436-9. pp.1436-9
Source: PubMed

ABSTRACT The purpose of this study was to evaluate lung cell injury during the acute phase of smoke inhalation injury. A group of 10 sheep were anesthetized with halothane and pancuronium followed by endotracheal intubation. In the first experiment 5 sheep were given air (sham group) and 5 were insufflated with cooled cotton smoke with a modified bee smoker. In the second part of our study (Experiment 2) the animals were insufflated with the following number of smoke breaths: 1 x 12 (n = 3); 2 x 12 (n = 4); 3 x 12 (n = 4) 4 x 12 (n = 4); and sham control (n = 1). After 30 min the animals were killed with KCl and the trachea prepared for scanning, transmission electron, and light microscopy. Our initial observation with scanning electron microscopy revealed a large amount of mucus on the surface of the epithelia. Numerous ciliated cells had been sloughed from the epithelium and were observed on the surface of the remaining ciliated cells. The sloughed cells were intact, and the cilia remained on the apical cell surface. Light and transmission electron microscopy revealed that most goblet cells were in the process of extruding mucus. The cytoplasm of goblet and basal cells appeared normal. Ciliated cells had a slightly vesiculated cytoplasm, and many were in the process of being sloughed from the epithelial surface. In these cells desmosomal attachment had been separated. The light microscope evaluation of the tracheal epithelium showed there was no dose-dependent effect between the four treatment groups.(ABSTRACT TRUNCATED AT 250 WORDS)

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Keywords

apical cell surface
 
basal cells
 
cells desmosomal attachment
 
Ciliated cells
 
cotton smoke
 
endotracheal intubation
 
epithelial surface
 
extruding mucus
 
first experiment 5 sheep
 
following number
 
goblet cells
 
modified bee smoker
 
Numerous ciliated cells
 
remaining ciliated cells
 
sham group
 
sloughed cells
 
smoke breaths
 
smoke inhalation injury
 
transmission electron
 
vesiculated cytoplasm
 

S. Abdi