Preparation of murine monoclonal antibodies against the yeast phase of the dimorphic fungus Sporothrix schenckii

Department of Clinical Sciences, Guy's Hospital, London Bridge, UK.
Transactions of the Royal Society of Tropical Medicine and Hygiene (Impact Factor: 1.84). 09/1990; 84(5):734-7. DOI: 10.1016/0035-9203(90)90169-F
Source: PubMed


Three murine monoclonal antibodies (Mabs) were raised against a cytoplasmic antigen of the yeast phase of the pathogenic fungus Sporothrix schenckii using a modification of standard hybridoma technology incorporating the immunosuppressive drug cyclophosphamide. When tested for species-specificity within the pathogenic dimorphic fungi one of these Mabs (S5) showed little cross-reactivity by enzyme-linked immunosorbent assay and Western blot, though there was some recognition of Paracoccidioides brasiliensis antigen. This Mab recognized a 70-75 kDa molecule on reduced Western blots of S. schenckii antigen. The other two Mabs (S12 and S15) showed cross-reactivity with all dimorphic fungal antigens tested, though they appeared to recognize a molecule of similar molecular weight. This is the first report of any attempt to raise species-specific Mabs against this important causative agent of dermatological disease.

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