Article

Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin.

Research Center for Veterinary Science, Kitasato Institute, Chiba, Japan.
FEMS Microbiology Letters (Impact Factor: 2.72). 02/1990; 54(1-3):39-43. DOI: 10.1016/0378-1097(90)90255-O
Source: PubMed

ABSTRACT Bordetella bronchiseptica dermonecrotic toxin was purified by a simplified method. The method consisted of SP Toyopearl 650M chromatography and high performance liquid chromatography on a TSK gel G3000SW column. 47.5% of the activity of the crude cell extract was recovered. The purified toxin behaved as a homogeneous protein in sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance liquid chromatography, and agar gel double diffusion tests.

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