Protective immunization against experimental Bacteroides (Porphyromonas) gingivalis infection.

Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
Infection and Immunity (Impact Factor: 4.16). 11/1990; 58(10):3394-400.
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ABSTRACT The effects of immunization in modulating the pathogenesis of Bacteroides (Porphyromonas) gingivalis infection in a murine model system were examined. BALB/c mice were immunized by intraperitoneal injection with B. gingivalis ATCC 53977 (one injection per week for 3 weeks), or with a lithium diiodosalicylate (LIS) extract (one injection per week for 3 weeks), or with lipopolysaccharide (LPS; one intravenous or intraperitoneal injection) from this same strain. Two weeks after the final immunization, the mice were challenged by subcutaneous injection of B. gingivalis ATCC 53977. Mice immunized with bacteria had no secondary lesions and no septicemia, whereas mice immunized with LIS extract had few secondary lesions and no septicemia. Mice immunized with LPS and nonimmunized mice demonstrated secondary abdominal lesions and septicemia after challenge. Bacterial cells and LIS extract, but not LPS, induced serum antibody and antigen reactive lymphocytes, as measured by enzyme-linked immunosorbent assay, immunoblot, Western immunoblot transfer, and in vitro lymphoproliferative responses. The present study suggests that immunization with a LIS extract or whole cells may induce a protective response against experimental B. gingivalis infection.

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    ABSTRACT: Porphyrononas gingivalis isa suspected pathogen inrapidly progressive periodontitis (RPP). We have determined theanti-P. gingivalis serum immunoglobulin G (IgG) isotype responseandavidity andthesubclass titer distributions for30RPP patients and30age-,sex-,andrace-matched healthy subjects byusing enzyme-linked immunosorbent assaytechnology. Patients andcontrol subjects were classified asseropositive if their total IgGresponsetoP.gingivalis was twofold ormore thanthemedian responseinhealthy subjects. The predominant antibody responsesforbothpatients andhealthy subjects were IgG2andIgG3, witha subclass order ofIgG2> IgG3> IgGl> IgG4. Theavidity oftheIgGresponsewas highest fortheseropositive healthy subjects andwas no different between seronegative andseropositive RPP patients. Thesubclass antibody responsesdidnotdepend on gender, andthere were no correlations between titer, avidity, or subclass with disease severity intheRPP patients as measuredbypocket depth or boneloss on dental X rays.The seronegative RPPpatients exhibited antibody responsesthat were greater thantheresponsesofseronegative healthy subjects forallfoursubclasses, while theseropositive RPPpatients hadhigher IgGlandIgG4levels thanseropositive healthy subjects. Thesefindings areconsistent withthehypothesis thatbothcarbohydrate and protein antigens areimportant intheIgGresponsetoP.gingivalis. Therelative predominance ofIgG2, a subclass whichlacks strong complement fixation andopsonic properties, andthelowavidity ofpatient anti-P. gingivalis IgGantibodies suggest thathumoral responsiveness toinfection withP.gingivalis may beineffective inclearing this organism. Periodontitis isa relatively common infectious disease leading totoothlossinadults worldwide. An unusual form characterized byan early ageofonset, involvement ofmost ifnotalloftheteeth, andarapid rateoftissue destruction is observed inyoung adults. Thisformhasbeendesignated rapidly progressive periodontitis (RPP) (4,32). Porphyromo- nasgingivalis isconsidered important intheetiology ofRPP (21,39).
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    ABSTRACT: A crude outer-membrane protein (OMP) preparation from a strain of Bacteroides fragilis, grown in supplemented brain-heart infusion broth, was tested for its protective effect against subcutaneous infection in mice. Immunization with six doses, each of 100, 150 or 200 μg OMP preparation, gave some protection: abscesses completely disappeared 15 to 22 days after immunization. In non-immunized animals and animals immunized with doses of 10, 20, 40 or 80 μg each, well demarcated abscesses were seen beyond day 22 post-immunization. Although crude OMP elicited good antibody response, with maximum titres on day 4 post-immunization, high titres could not be associated with healing of the abscesses.
    World Journal of Microbiology and Biotechnology 11/1994; 10(6):645-7. DOI:10.1007/BF00327951 · 1.35 Impact Factor
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