Heterogeneity of human peripheral blood Eo-type colonies: evidence for a common eosinophil-basophil progenitor

Blood (Impact Factor: 10.45). 09/1985; 66(2):312-8.
Source: PubMed


We have recently shown that a proportion of previously designated human eosinophil "(Eo)-type" colonies in methylcellulose contain basophils and histamine (Denburg et al Blood 61:775, 1983). In the present studies, individual Eo-type colonies have been analyzed by cell morphology as well as by biochemical assays for histamine, Charcot-Leyden crystal protein (CLC), and eosinophil granule major basic protein (MBP). Clonal origin of single Eo-type colonies was confirmed by G6PD isoenzyme analysis. Morphological observations of such colonies revealed the existence of two distinct colony types: (1) Eo type containing 100% basophils and (2) Eo type containing mixtures of basophils and eosinophils, including cells with mixed basophil-eosinophil granulation. Histamine was not detected in pure, mature peripheral blood eosinophils. Immunofluorescent studies demonstrated bright staining for CLC and MBP in 95% +/- 3% of cells in Eo-type colonies but only in 5% +/- 4% of cells in GM-type colonies. Radioimmunoassay for MBP was positive in 5/9 Eo-type and 0/10 neutrophil-macrophage ("GM-type") colonies, with a mean level (nanogram/colony) of 11.6 +/- 4.2 per Eo-type colony; four of the latter colonies were doubly positive for both histamine and MBP. These and previous findings point out the morphological and biochemical heterogeneity of peripheral blood Eo-type colonies and provide direct evidence for the existence of a common, circulating basophil-eosinophil progenitor.

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    • "This shows that although the cells, by staining and cell surface marker expression, resemble immature basophils they are more eosinophil-like when analyzing the transcriptome. This mixed phenotype further strengthens the old notion that in humans eosinophils and basophils are closely linked during development [42], [43], [44]. Interestingly, this situation does apparently not apply for murine basophils where basophils and mast cells seem to have a common precursor [45], [46], [47]. "
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    ABSTRACT: Basophils are blood cells of low abundance associated with allergy, inflammation and parasite infections. To study the transcriptome of mature circulating basophils cells were purified from buffy coats by density gradient centrifugations and two-step magnetic cell sorting. However, after extensive analysis the cells were found to be transcriptionally inactive and almost completely lack functional mRNA. In order to obtain transcriptionally active immature basophils for analysis of their transcriptome, umbilical cord blood cells were therefore cultured in the presence of interleukin (IL)-3 for 9 days and basophils were enriched by removing non-basophils using magnetic cell sorting. The majority of purified cells demonstrated typical metachromatic staining with Alcian blue dye (95%) and expression of surface markers FcεRI and CD203c, indicating a pure population of cells with basophil-like phenotype. mRNA was extracted from these cells and used to construct a cDNA library with approximately 600 000 independent clones. This library served as tool to determine the mRNA frequencies for a number of hematopoietic marker proteins. It was shown that these cells express basophil/mast cell-specific transcripts, i.e. β-tryptase, serglycin and FcεRI α-chain, to a relatively low degree. In contrast, the library contained a high number of several eosinophil-associated transcripts such as: major basic protein (MBP), charcot leyden crystal (CLC), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO). Out of these transcripts, MBP and EPO were the most frequently observed, representing 8% and 3.2% of the total mRNA pool, respectively. Moreover, in a proteome analysis of cultured basophils we identified MBP and EPO as the two most prominent protein bands, suggesting a good correlation between protein and mRNA analyses of these cells. The mixed phenotype observed for these cells strengthens the conclusion that eosinophils and basophils are closely linked during human hematopoietic development. The dual phenotype also indicates that other cytokines than IL-3 or cell surface interactions are needed to obtain the full basophil specific phenotype in vivo.
    PLoS ONE 10/2012; 7(10):e48308. DOI:10.1371/journal.pone.0048308 · 3.23 Impact Factor
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    • "Eosinophils are derived from multipotent and lineage-committed hematopoietic stem cells [1]. The major growth factors for eosinophils are interleukin (IL)-3, IL-5, and granulocyte monocyte-colony stimulating factor (GM-CSF). "
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    Cancer Research and Treatment 09/2011; 43(3):199-203. DOI:10.4143/crt.2011.43.3.199 · 3.32 Impact Factor
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    • "Our group and others have illustrated the involvement of Eo/B progenitors in allergic inflammatory disease in the following studies: i) increases in circulating Eo/B CFU in subjects with atopy and allergic rhinitis [1,4]; ii) increases in circulating Eo/B CFU during seasonal exposure to allergens [5,6]; iii) increases in circulating and bone marrow progenitors and upregulation of IL-5Rα expression after allergen inhalation challenge [7-10] and iv) increases in airway CD34+ cells expressing the IL-5Rα after allergen inhalation [11]. "
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    The Open Respiratory Medicine Journal 02/2008; 2(1):60-6. DOI:10.2174/1874306400802010060
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