The cell surface molecule recognized by the erythrocyte receptor of T lymphocytes. Identification and partial characterization using a monoclonal antibody

Journal of Experimental Medicine (Impact Factor: 12.52). 10/1985; 162(3):890-901. DOI: 10.1084/jem.162.3.890
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A monoclonal antibody (mAb) to sheep red blood cells (SRBC), termed L180/1, is described that completely blocks rosette formation between SRBC and human or sheep T lymphocytes. L180/1 precipitated a minor glycoprotein of about approximately 42,000 mol wt from surface-labeled SRBC. This glycoprotein was partially affinity purified and found to block E rosette formation and to compete with anti-T11 mAb for the E receptor. The molecule detected by mAb L180/1 thus appears to be recognized by the E receptor and was given the preliminary name, T11 target structure (T11TS). Since the mAb to sheep T11TS blocks the binding of SRBC to both human and sheep T cells, and mAb to T11 blocks the binding of red cells from human and sheep to the human E receptor, we concluded that analogous receptor-ligand (T11-T11TS) systems exist in man and sheep that are crossreactive over the species barrier. The possibility is discussed that the E receptor, which is known to be involved in T cell activation, and T11TS function as complementary cell interaction molecules in T cell responses.

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Available from: Thomas Hünig, Oct 03, 2015
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    • "The E-rosette-forming capacity has been reported in human and murine T cells of both gd and ab lineages, and porcine T cells of the ab and CD2 + gd lineages (Licence and Binns, 1984; Hunig, 1985; Kontny and Ryzewska, 1989; Binns, 1994). E-rosette formation is the result of the interaction between CD2 molecules on T cells and their targets on xenogeneic RBC (Hunig, 1985; Tiefenthaler et al., 1987). In contrast, porcine CD2 7 gd T cells do not have the E-rosette-forming capacity and thus are referred to as the non-classical T cells of pigs (Saalmuller et al., 1990; Binns et al., 1992; Binns, 1994). "
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    ABSTRACT: The present review concentrates on the biological aspects of porcine T lymphocytes. Their ontogeny, subpopulations, localization and trafficking, and responses to pathogens are reviewed. The development of porcine T cells begins in the liver during the first trimester of fetal life and continues in the thymus from the second trimester until after birth. Porcine T cells are divided into two lineages, based on their possession of the alphabeta or gammadelta T-cell receptor. Porcine alphabeta T cells recognize antigens in a major histocompatibility complex (MHC)-restricted manner, whereas the gammadelta T cells recognize antigens in a MHC non-restricted fashion. The CD4+CD8- and CD4+CD8lo T cell subsets of alphabeta T cells recognize antigens presented in MHC class II molecules, while the CD4-CD8+ T cell subset recognizes antigens presented in MHC class I molecules. Porcine alphabeta T cells localize mainly in lymphoid tissues, whereas gammadelta T cells predominate in the blood and intestinal epithelium of pigs. Porcine CD8+ alphabeta T cells are a prominent T-cell subset during antiviral responses, while porcine CD4+ alphabeta T cell responses predominantly occur in bacterial and parasitic infections. Porcine gammadelta T cell responses have been reported in only a few infections. Porcine T cell responses are suppressed by some viruses and bacteria. The mechanisms of T cell suppression are not entirely known but reportedly include the killing of T cells, the inhibition of T cell activation and proliferation, the inhibition of antiviral cytokine production, and the induction of immunosuppressive cytokines.
    Animal Health Research Reviews 06/2006; 7(1-2):81-96. DOI:10.1017/S1466252307001235
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    • "One of the first cell adhesion systems to be defined and reconstituted was based on the interaction of CD2 and CD58. Monoclonal antibody inhibition studies pointed to an adhesion mechanism based on the interaction of CD2 (also called T11, sheep red blood cell receptor and LFA-2) with CD58 (also called T11 target structure and LFA-3) (Hünig, 1985; Shaw et al., 1986; Vollger et al., 1987). CD2 –CD58 interactions were directly demonstrated using purified CD2 and purified CD58, the later reconstituted in supported planar bilayers (Dustin et al., 1987a; Selvaraj et al., 1987). "
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    ABSTRACT: Supported planar bilayers have been used extensively in immunology to study molecular interactions at interfaces as a model for cell-cell interaction. Examples include Fc receptor-mediated adhesion and signaling and formation of the immunological synapse between T cells and antigen-presenting cells. The advantage of the supported planar bilayer system is control of the bilayer composition and the optical advantages of imaging the cell-bilayer or bilayer-bilayer interface by various types of trans-, epi- and total internal reflection illumination. Supported planar bilayers are simple to form by liposome fusion and recent advances in micro- and nanotechnology greatly extend the power of supported bilayers to address key questions in immunology and cell biology.
    Journal of Immunological Methods 08/2003; 278(1-2):19-32. DOI:10.1016/S0022-1759(03)00193-5 · 1.82 Impact Factor
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