Digestion of killed Paracoccidioides brasiliensis by neutrophils
ABSTRACT We previously described an in vitro assay showing that neutrophils (PMNs) from patients with paracoccidioidomycosis (PARA) have a specific digestive deficiency against suspensions of live Paracoccidioides brasiliensis. We now report that this defect is equally detectable against autoclaved, but not Amphotericin B-killed P. brasiliensis. The use of autoclaved suspensions facilitates the use of our in vitro assay. It might allow the development of an in vitro intradermal test for digestion of fungi. Differential digestive ability of phagocytes against live (or autoclaved) and Amphotericin-B killed fungi is of conceptual interest. It may be relevant in understanding therapeutic effect of Amphotericin B.
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ABSTRACT: Paracoccidioidomycosis is a human systemic mycosis caused by the thermally dimorphic fungus Paracoccidioides brasiliensis. Geographically, it is confined to Latin America with its endemic areas extending from Central America down to Argentina, constituting one of the most prevalent deep mycoses in this region [44,83]. Reviews covering partial aspects of the subject have been published from time to time [44,81 83,91,92], and for this reason this review will concentrate mainly on papers published from 1989 onwards, with only brief mention of earlier works.Journal of medical and veterinary mycology: bi-monthly publication of the International Society for Human and Animal Mycology 02/1993; 31(2):99-113. DOI:10.1080/02681219380000121
- International Journal of Dermatology 06/1994; 33(5):337-40. DOI:10.1111/j.1365-4362.1994.tb01064.x · 1.31 Impact Factor
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ABSTRACT: Paracoccidioidomycosis is a systemic granulomatous disease that involves primarily the lungs and may disseminate to other organs and systems. It is caused by Paracoccidioides brasiliensis, a fungus that exhibits reversible thermal dimorphism and whose natural habitat is presently unknown. There are two main clinical forms: the acute (subacute) juvenile form and the chronic adult form. The former runs a more rapid course and is more severe than the latter. This mycosis is found throughout Latin America. Brazil accounts for 80% of reported cases. Presumably P. brasiliensis thrives in humid and hot places, especially near forests or farms. The infection is endemic in certain areas, especially in Brazil, Colombia and Venezuela, where nearly 100% of the population show cutaneous paracoccidioidina positive skin tests, indicating previous contact with the fungus, although a small percentage show clinical manifestations of the disease. We compared the expression of HLA class I antigens in a healthy group (control) and in a group of patients with paracoccidioidomycosis (chronic adult form) using the Terasaki lymphocytotoxicity test modified by Amos for HLA antigen analysis. To discover indications of whether or not individual susceptibility to P. brasiliensis might depend on some specific immunological defect. There is no evidence of association between a specific HLA antigen and paracoccidioidomycosis in the subjects studied. Further investigations are recommended.Journal of the European Academy of Dermatology and Venereology 06/2000; 14(3):166-71. DOI:10.1046/j.1468-3083.2000.00070.x · 2.83 Impact Factor