Cigarette smoke stimulates cathepsin B activity in alveolar macrophages of rats.

Tobacco and Health Research Institute, University of Kentucky, Lexington, KY 40546-0236.
Journal of Laboratory and Clinical Medicine (Impact Factor: 2.8). 11/1989; 114(4):419-25.
Source: PubMed


Cathepsin B activity was determined in alveolar macrophages and cell-free bronchoalveolar lavage fluid from Sprague-Dawley rats exposed only through the nose to fresh mainstream smoke from University of Kentucky high-tar, high-nicotine reference cigarettes, and in cells and fluid from room control and sham control animals. Increased levels of blood carboxyhemoglobin and pulmonary aryl hydrocarbon hydroxylase activity in smoke-exposed animals indicated effective exposure of animals to cigarette smoke. Cathepsin B activity was quantitated with alpha-N-benzyloxycarbonyl-leucine-leucine-arginine-2-naphthylamide as substrate. Specific activity (nanomoles of substrate cleaved per milligram of protein per hour) in alveolar macrophages was increased by 43% at both 4- and 10-week exposure points in animals exposed twice daily to 10 puffs of cigarette smoke. These data indicate that maximal stimulation of the enzyme occurs within 4 weeks of the initiation of smoke exposure. When the activity was expressed on a per-cell basis, cathepsin B activity was also increased in the smoke-exposed group at both exposure points. Activity in bronchoalveolar lavage fluid of smoke-exposed animals was increased by approximately 50% at 4 and 10 weeks, but the differences were not statistically significant. These findings demonstrate that cigarette smoke is a potent inducer of cathepsin B activity in alveolar macrophages of rats.

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    • "These effects may be due to the conversion of carcinogens by the cytochrome P450 enzymes CYP1a1 and CYP1b1 to metabolites that can bind to TNF-α receptors (Nagaraj et al., 2006a). Our findings are in agreement with prior studies which showed that cathepsin expression and activity were upregulated by cigarette smoke treatment of pulmonary macrophages, dendritic cells, or alveolar macrophages (Chang et al., 1989;Gairola et al., 1989;Bracke et al., 2005). "
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