Spatial Programming of Gene Expression in Early Drosophila Embryogenesis

Annual Review of Cell Biology 02/1986; 2(1):49-80. DOI: 10.1146/annurev.cb.02.110186.000405
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Available from: Patrick H O'Farrell,
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    • "The segmentation process in Drosophila is controlled by a set of —25 regulatory genes that are active in the early embryo (for review, see Akam 1987; Ingham 1988). Localized expression of the segmentation genes depends on a hierarchy of regulatory interactions (for review, see Scott and OTarrell 1986). The first step is the establishment of broadly distributed gradients of maternal morphogens during oogenesis. "
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    ABSTRACT: We have studied the ability of the Drosophila gap proteins Krüppel and hunchback to function as transcriptional regulators in cultured cells. Both proteins bind to specific sites in a 100-bp DNA fragment located upstream of the segment polarity gene engrailed, which also contains functional binding sites for a number of homeo box proteins. The hunchback protein is a strikingly concentration-dependent activator of transcription, capable of functioning both by itself and also synergistically with the pair-rule proteins fushi tarazu and paired. In contrast, Krüppel is a transcriptional repressor that can block transcription induced either by hunchback or by several different homeo box proteins. While repression of the homeo box protein activators requires a Krüppel-binding site on the DNA, repression of hunchback can occur efficiently in the absence of a Krüppel-binding site. We discuss the possible molecular mechanisms underlying these activities, as well as the potential significance of these results with respect to segmentation in Drosophila.
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    ABSTRACT: To identify candidates for neuronal recognition molecules in Drosophila, we used monoclonal antibodies to search for surface glycoproteins expressed on subsets of axon bundles (or fascicles) during development. Here we report on the characterization and cloning of fasciclin III, which is expressed on a subset of neurons and axon pathways in the Drosophila embryo. Fasciclin III is also expressed at other times and places including transient segmentally repeated patches in the neuroepithelium and segmentally repeated stripes in the body epidermis. Antisera generated against each of four highly related forms of the protein were used for cDNA expression cloning to identify a single gene, which was confirmed to encode fasciclin III by tissue in situ hybridization and genetic deficiency analysis.
    Cell 04/1987; 48(6):975-88. DOI:10.1016/0092-8674(87)90706-9 · 32.24 Impact Factor
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    ABSTRACT: We report here that a previously described cell surface antigen (Brower, Smith & Wilcox, 1980) is expressed in a segmentally repeating pattern of stripes in the epidermis and nervous system of segmented Drosophila embryos. We also report that the antigenic activity is found on two closely related cell surface glycoproteins. The pattern of expression of this antigen is reminiscent of the expression of some segmentation genes and is affected by mutation of at least two of these genes, fushi tarazu and paired. Thus these glycoproteins are candidates for cell surface molecules involved in carrying out the patterning processes controlled by segmentation genes.
    Development 06/1987; 100(2):237-44. · 6.46 Impact Factor
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