Article

Purification and characterization of mouse hematopoietic stem cells

Stanford University, Palo Alto, California, United States
Science (Impact Factor: 31.48). 08/1988; 241(4861):58-62. DOI: 10.1126/science.2898810
Source: PubMed

ABSTRACT Mouse bone marrow hematopoietic stem cells were isolated with the use of a variety of phenotypic markers. These cells can
proliferate and differentiate with approximately unit efficiency into myelomonocytic cells, B cells, or T cells. Thirty of
these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the
survivors.

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    • "d hematopoietic cells ( Till and McCulloch , 1961 ; Spangrude , 1989 ) . Several cell surface molecules have been identified ( Shinohara et al . , 2000 ; Kubota et al . , 2003 ) that allow for the enrichment of the mouse SSC for transplantation and study . Interestingly , some of these molecules have been identified on hematopoietic and ES cells ( Spangrude et al . , 1988 ; Williams et al . , 1991 ; Xu et al . , 2001 ) , suggesting similar molecular characteristics across stem cell populations . However , little is known about the cell surface phenotype of SSC in nonrodent mammals including agriculturally valuable livestock such as pigs due to the lack of techniques to distinguish and isolate the SSC fro"
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    • "This article can be cited as DOI: 10.1369/0022155414520710 12 however cells expressing only one of the two markers Sca1 and CD45 are unlikely to be HSCs. Sca1 is a member of the Ly6A superfamily of glycerophosphatidylinositol (GPI)anchored membrane proteins (Holmes and Stanford 2007), involved in stem cell selfrenewal , fate decisions, lipid raft signaling via weak protein-protein interaction in HSCs (Holmes and Stanford 2007; Okada et al. 1992; Spangrude et al. 1988). The CD45 is a hematopoietic cell marker presented on the surface of HSCs, it plays a critical role in survival, expansion, differentiation, and migration of HSCs during process of hematopoiesis (Kiel and Morrison 2008; Raaijmakers and Scadden 2008). "
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    ABSTRACT: Various types of endogenous stem cells (SCs) participate in wound healing in the skin at different anatomical locations. SCs need to be identified through multiple markers, and this is usually performed using flow cytometry. However, immunohistological identification of endogenous stem cells in the skin at different anatomical locations by co-staining multiple SC markers has been seldom explored. We examined the immunohistological localization of four major types of SCs in wounded skin by co-staining for their multiple markers. Hematopoietic SCs were co-stained for Sca1 and CD45; mesenchymal SCs for Sca1, CD29, and CD106; adipose SCs for CD34, CD90, and CD105; and endothelial progenitor cells and their differentiated counterparts were co-stained for CD34, Tie2, and von Willebrand factor. We found Sca1(+)CD45(+) SCs in the epidermis, dermis and hypodermis of wounded skin. Sca1(+)CD29(+) and Sca1(+)CD106(+) mesenchymal SCs, CD34(+)CD105(+), CD34(+)CD90(+), and CD90(+)CD105(+) adipose SCs, as well as CD34(+)Tie2(+) endothelial progenitor cells were also located in the epidermis, dermis, and hypodermis. This study demonstrates the feasibility of using immunohistological staining to determine the location of SCs in wounded skin and the intracellular distribution of their molecular markers.
    Journal of Histochemistry and Cytochemistry 01/2014; 62(4). DOI:10.1369/0022155414520710 · 2.40 Impact Factor
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    • ". Functional studies involving adoptive transfer of HSC to reconstitute the hematopoietic system of lethally irradiated mice, remains the gold standard assay to distin‐ guish ST-HSC from LT-HSC [18]. While LT-HSC are able to sustain reconstitution of the hematopoietic system over more than 25 weeks, and even a lifetime [19], ST-HSC provide short-term reconstitution for only ~6 weeks [17] "
    Adult Stem Cell Niches, Edited by Dr Sabine Wislet-Gendebien (Ed, 01/2014: chapter 1: pages 4 - 20; Intech Open Publishing., ISBN: 978-953-51-1718-6
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