Article

Transformation of dog embryo kidney cells by human herpesviruses.

Cell Biology International Reports 08/1986; 10(7):493-9. pp.493-9
Source: PubMed

ABSTRACT The infection of dog embryo kidney (DEK) cells with herpes simplex virus type 2 (HSV-2) or human cytomegalovirus (HCMV) led to the development of transformed cell lines. Rapidly dividing DEK cells with unlimited division potential exhibited growth in 2% serum, contained nuclear virus antigens, and formed small (+/- 0.2 mm) colonies in 0.3% agarose. Immortal cell lines showing the same transformation properties were also obtained after transfection with purified HSV-2 or HCMV DNA. These results confirm the transforming capacity of both herpesviruses as well as the usefulness of this different type of mammalian cells in transformation studies.

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Keywords

cell lines
 
dog embryo kidney
 
herpes simplex virus type 2
 
herpesviruses
 
human cytomegalovirus
 
Immortal cell lines
 
mammalian cells
 
purified HSV-2
 
transformation properties
 
transformation studies
 
transforming capacity
 
unlimited division potential exhibited growth
 
usefulness
 

J Yelle