Article

Periportal and perivenous hepatocytes retain their zonal characteristics in primary culture.

Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark
Biochemical and Biophysical Research Communications (Impact Factor: 2.28). 10/1986; 139(3):1055-61. DOI: 10.1016/S0006-291X(86)80284-4
Source: PubMed

ABSTRACT Periportal and perivenous hepatocytes from rat liver were isolated by combined digitonin-collagenase perfusion, and gluconeogenesis, urea synthesis and fatty acid synthesis was measured both in freshly isolated cells and in primary culture. A periportal zonation of gluconeogenesis and urea synthesis of about 3 and 1.5 fold, respectively, was observed. This zonation persisted unchanged for 23 hours in culture under identical conditions of incubation for periportal and perivenous cells. Fatty acid synthesis was not zonated.

0 Bookmarks
 · 
41 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The liver is well recognized as a target for injury during low flow or inflammatory states. Functionally, the result is both metabolic and host defense dysfunction. Although the liver is clearly responsive to changes in systemic levels of various mediators, it is becoming apparent that substantial changes occur within the liver that are not directly dependent on extrahepatic factors. This is the result of complex interactions among the various cell types that exist in a highly organized arrangement within the functional subunit of the liver. The purpose of this review is to summarize the structural relationships which form the basis for this system of cell-cell communication and their functional implications both in the normal liver and during both low-flow and normal-flow inflammatory states. (C)1994The Shock Society
    Shock 06/1994; 2(1). DOI:10.1097/00024382-199407000-00001 · 2.73 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Periportal and perivenous hepatocytes were isolated from rat liver by digitonin/collagenase perfusion for investigating the acinar distribution of taurocholate uptake. Statistical analysis revealed that uptake of taurocholate by periportal, perivenous and regular (whole acinus) hepatocytes in Na+-containing and -free buffer was best described by one saturable component. Total taurocholate uptake measured in Na+-containing buffer was significantly higher in perivenous (Vmax=7.5 nmol/(min mg protein)) than in periportal hepatocytes (Vmax=5.4 nmol/(min mg protein)). Uptake by regular hepatocytes was well between the values of periportal and perivenous hepatocytes (Vmax=6.7 nmol/(min mg protein)). The Km-values were not different among the zonal regions. In Na+-free buffer, Km and Vmax of taurocholate uptake calculated for all fractions were similar. During cultivation of hepatocytes as monolayer total taurocholate uptake strongly decreased and the zonal differences observed in freshly isolated cells in suspension disappeared. Initial uptake rates of Na+-independent taurocholate uptake and the ATP-content of the hepatocytes were constant. Our results indicate an acinar gradient of Na+-dependent taurocholate uptake activity, which may improve the clearance of bile salts from portal blood and protect periportal hepatocytes against a too high intracellular bile salt concentration.
    Hepatology Research 02/2004; 28(2):114–123. DOI:10.1016/j.hepres.2003.12.005 · 2.07 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A pronounced heterogeneity between hepatocytes in subcellular structure and enzyme activities was discovered more than 50 years ago and initiated the idea of metabolic zonation. In the last decades zonation patterns of liver metabolism were extensively investigated for carbohydrate, nitrogen and lipid metabolism. The present review focuses on zonation patterns of the latter. We review recent findings regarding the zonation of fatty acid uptake and oxidation, ketogenesis, triglyceride synthesis and secretion, de novo lipogenesis, as well as bile acid and cholesterol metabolism. In doing so, we expose knowledge gaps and discuss contradictory experimental results, for example on the zonation pattern of fatty acid oxidation and de novo lipogenesis. Thus, possible rewarding directions of further research are identified. Furthermore, recent findings about the regulation of metabolic zonation are summarized, especially regarding the role of hormones, nerve innervation, morphogens, gender differences and the influence of the circadian clock. In the last part of the review, a short collection of models considering hepatic lipid metabolism is provided. We conclude that modeling, despite its proven benefit for understanding of hepatic carbohydrate and ammonia metabolisms, has so far been largely disregarded in the study of lipid metabolism; therefore some possible fields of modeling interest are presented.
    Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 02/2015; DOI:10.1016/j.bbalip.2015.02.004 · 4.50 Impact Factor