Methods for assaying nonenzymatic glycosylation.

Open University, Oxford Research Unit, England.
Analytical Biochemistry (Impact Factor: 2.58). 01/1989; 175(2):347-60. DOI: 10.1016/0003-2697(88)90558-1
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    ABSTRACT: Glycation is a common modification of proteins both in vitro and in vivo. To aid identification and comparative quantitation, a method of stable isotope labeling followed by LC-MS analysis was proposed. The samples were reduced using sodium borohydride or sodium borodeuteride. Reduction of the Schiff base between the amine group and the reducing sugars resulted in a molecular weight increase of 2Da using sodium borohydride or a molecular weight increase of 3Da using sodium borodeuteride. The molecular weight difference of 1Da between peptides containing glycated lysine residue reduced using sodium borohydride or sodium borodeuteride was used to identify glycated peptides and to calculate the glycation difference between samples. The method was used to investigate glycation of a recombinant human IgG1 antibody under native and denaturing conditions. The result demonstrated a good correlation between glycation propensity of lysine residues and their solvent exposure levels.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 03/2014; 958C:90-95. · 2.78 Impact Factor
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    IJCBS. 01/2014;
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    ABSTRACT: We examined effects of red ginseng on the formation of glycated protein in vivo and in vitro. The mixtures (1 : 1 : 1, v/v/v) with glucose (1.5 g/dl, hemoglobin (10 g/d) and red ginseng extract (0.5 g/dl) in 0.067 M phosphate saline buffer were incubated for 5 days in shaking water bath (37, 70 RPM). Male rats were divided into three groups with one health and two diabetes, consisting of 20 heads in each group. Diabetic rats, induced by streptozotocin injection, were treated with or without red ginseng extract (100 mg/kg/day) for 3 months. The concentration of blood glucose and the rate of glycated hemoglobin were determined by commercial kits. The rate of glycated hemoglobin was significantly decreased by the addition of ginseng extract in comparison with non-addition group in vitro (12.17 1.01% vs 15.9 1.95%, meansd, p
    Journal of ginseng research 01/2002; 26(4). · 2.26 Impact Factor