Phylogenies from whole genomes: Methodological update within a distance-based framework

Methods which derive pairwise distances directly from complete sequenced genomes are a potentially important and efficient tool within the growing field of phylogenomics. We have shown in two previous studies that the Genome BLAST Distance Phylogeny (GBDP) approach leads to reliable phylogenetic estimates if applied to prokaryotic as well as plastid and mitochondrial genomes. Basically, GBDP first invokes tools such as BLAST to identify high-scoring segment pairs (HSPs) between all pairs of genomes; afterwards, pairwise distances are estimated based on different formulae.
Here, we examine (1) a new GBDP distance formula, based on a combination of two previously existing ones; (2) use of BLAT instead of BLASTN and TBLASTX HSP search; (3) an alternative measure for the agreement of a distance matrix with a predefined reference topology; (4) alternative topology-independent measures of distance quality per se. All examinations were based on a enlarged dataset compared to that used in our previous study, additionally containing interesting key taxa.