Analysis of genetic diversity among wild pomegranates in Western Himalayas, using PCR methods
ABSTRACT The genus Punica (Punicaceae) is distributed in Iran, Afghanistan, India and Mediterranean countries. Iran is considered to be its primary center of origin. In India Punica granatum is found in wild only in Western Himalayan regions comprising, Jammu and Kashmir, Himachal Pradesh and Uttarakhand states.
However, there is little information available about the genetic variation present in pomegranates in the regions. In this paper we describe the use ofDAMD and RAPD methods that generate the profiles, to study genetic diversity in wild genotypes of the P. granatum in India. Forty-nine accessions representing two regions of Western Himalaya were analyzed. Similarity coefficient value varied from 0.08 to 0.79 across different accessions. The results indicate that DAMD (97.08%) revealed more polymorphism in comparison to RAPD (93.72%). The results show that these methods are sufficiently informative to unravel the genetic variations in wild pomegranates in Western Himalayas.
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ABSTRACT: A strategy based on random PCR amplification was used to isolate new repetitive elements of Arabidopsis thaliana. One of the random PCR product analyzed by this approach contained a tandem repetitive minisatellite sequence composed of 33 bp repeated units. The genomic locus corresponding to this PCR product was isolated by screening a lambda genomic library. New related loci were also isolated from the genomic library by screening with a 14 mer oligonucleotide representing a region conserved among the different repeated units. Alignment of the consensus sequence for each minisatellite locus allowed the definition of an Arabidopsis thaliana core sequence that shows strong sequence similarities with the human core sequence and with the generalized recombination signal Chi of Escherichia coli. The minisatellites were tested for their ability to detect polymorphism, and their chromosomal position was established.Nucleic Acids Research 09/1994; 22(16):3317-21. · 8.28 Impact Factor
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ABSTRACT: A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice.Theoretical and Applied Genetics 01/1997; 95(5):942-949. · 3.66 Impact Factor
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ABSTRACT: A total of 373 species of wild relatives of crop plants representing 120 genera and 48 families were collected from different phytogeographical regions of India during a mission mode sub-project on Sustainable Management of Plant Biodiversity under National Agricultural Technology Project during 1999–2005. Significant diversity representing endemic/rare/endangered species has been discussed in this paper. The information on phytogeographical distribution, life form, economic types and assessment of threat has also been included. Thrust areas for future target collections and conservation have been discussed to serve as reference for management of genetic resources.Genetic Resources and Crop Evolution 02/2008; 55(2):187-202. · 1.59 Impact Factor