Antibody response to serogroup B meningococcal outer membrane antigens after vaccination and infection

National Institute of Public Health, Oslo, Norway.
Journal of Clinical Microbiology (Impact Factor: 3.99). 09/1988; 26(8):1543-8.
Source: PubMed


Antibody responses of adult volunteers given a vaccine containing meningococcal capsular polysaccharides (serogroups A, C, Y, and W-135) noncovalently complexed with serotype 2b:P1.2 and 15:P1.16 outer membrane proteins have been studied. Sera were analyzed by enzyme-linked immunosorbent assay methods for immunoglobulin G (IgG), IgM, and IgA antibodies and for bactericidal activities against the homologous strains. The vaccination was performed as a double-blind experiment with 47 volunteers, of whom 23 received the protein-polysaccharide vaccine and 24 received the control preparation containing the polysaccharides only. Ten additional persons volunteered for the protein-polysaccharide vaccine. Before vaccination, carriers of meningococci had significantly higher levels of specific IgG and IgA and also higher bactericidal activities than noncarriers. At 2 weeks postvaccination we found significant IgG and bactericidal antibody responses against both the 2b:P1.2 and 15:P1.16 strains in about 70% of the protein-polysaccharide vaccinees. The immune response induced by disease was compared with that induced by vaccination by analyzing paired sera from 13 survivors of serogroup B serotype 15 meningococcal disease. We found that the mean specific IgG level in acute-phase sera was lower than average in prevaccination sera from the vaccinees but similar to that of healthy noncarriers before vaccination. The convalescent-phase sera showed IgG responses similar to those of the vaccinees, but the IgM response to disease was significantly higher than after vaccination. The immune response to disease caused by serogroup B serotype 15 meningococci was found by enzyme-linked immunosorbent assay analysis to be about the same with outer-membrane antigens from a serotype 2b strain as it was with antigens from a serotype 15 strain.

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Available from: Stig Harthug, Oct 06, 2015
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    • "Outer membrane proteins (OMP) have been shown to be immunogenic and are being assessed for their potential as vaccine candidates. IgG antibody levels to serogroup B OMP antigens were signi¢cantly higher for carriers before and after immunization with the meningococcal vaccine under investigation in Norway [6]. The objective of the current study was to use ELISA to detect IgM and IgG antibodies speci¢c for OMP from isolates expressing phenotypes associated with meningococcal disease in Greece to determine if carriage of meningococci or N. lactamica was associated with induction of antibodies to these antigens. "
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    ABSTRACT: Carriage of non-serogroupable Neisseria meningitidis or Neisseria lactamica induces antibodies protective against meningococcal disease. Antibodies directed against outer membrane proteins are bactericidal and the serotype and subtype outer membrane protein antigens are being examined for their value as vaccine candidates for serogroup B disease. The aim of this study was to examine the effect of carriage of these two Neisseria species among children and young adults on induction of antibodies to outer membrane components from strains causing disease in Greece. Among 53 patients with meningococcal disease, IgG or IgM antibodies were detected by ELISA in 9 of 13 (69%) from whom the bacteria were isolated and 27 of 40 (67%) who were culture-negative. For military recruits (n = 604), the proportion of carriers of meningococci with IgM or IgG to outer membrane proteins was higher than non-carriers, P < 0.05 and P = 0.000000, respectively. Among school children (n = 319), the proportion with IgM or IgG to outer membrane proteins for carriers of meningococci was higher compared with non-carriers, P = 0.000000 and P = 0000043, respectively. Carriage of N. lactamica was not associated with the presence of either IgM or IgG to the outer membrane proteins in the children. The higher proportion of children (50%) with IgM to outer membrane proteins compared with recruits (10%) might reflect more recent exposure and primary immune responses to the bacteria. The lack of association between antibodies to outer membrane proteins and carriage of N. lactamica could reflect observations that the majority of N. lactamica isolates from Greece and other countries do not react with monoclonal typing reagents. Bactericidal antibodies to meningococci associated with high levels of IgG to N. lactamica were found in a previous study; these are thought to be directed to antigens other than outer membrane proteins or capsules and imply antigens such as lipo-oligosaccharide are involved in induction of antibodies cross-reactive with meningococci.
    FEMS Immunology & Medical Microbiology 05/1999; 24(1):73-8. DOI:10.1016/S0928-8244(99)00009-7 · 3.08 Impact Factor
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    ABSTRACT: Tiivistelmä. Diss. -- Helsingin yliopisto.
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    ABSTRACT: We havedetermined theamountsofspecific anti-class 1outer membraneprotein antibodies inserafrom25 patients during thecourse ofsystemic meningococcal disease, using purified class 1protein asthesensitizing antigen inanenzyme-linked immunosorbent assay. Theclass 1protein wasobtained fromavariant ofstrain 44/76 (B:15:P1.7,16) lacking class 3andclass 4outermembraneproteins. Specific anti-class 1(serosubtype P1.7,16) outermembraneprotein immunoglobulin G (IgG)antibody levels increased significantly in12 patients (12of25;48%),regardless oftheserotype oftheinfecting strain, indicating thattheantibodies reacted inpartwithepitopes notdetermined bythemonoclonal antibodies usedforserotyping. Mostpatients hadlow levels ofanti-class 1IgGantibodies during theacute illness. Theantibody levels peaked during thesecond week ofdisease andreturned tonearbaseline levels inseracollected 6weeksto12monthsafter theonsetofthe disease. Themajority ofthespecific anti-class 1IgGantibodies boundtosurface-exposed epitopes onwhole bacteria andbelonged totheIgGlandIgG3subclasses. Anti-class 1IgAandIgMantibodies werenotdetected inanyofthepatient sera. Prior todisease, sevenpatients hadbeenimmunized withameningococcal outer membranevesicle vaccine developed fromstrain 44/76(P1.7,16). Noneofthesepatients wasinfected with meningococcal strains containing class 1protein homologous orpartly homologous tothatofthevaccine strain, indicating serosubtype-specific protection. Thehighest anti-class 1IgGantibody peaklevels wereseen inimmunized patients infected withstrains ofheterologous serotype, suggesting ananamnestic response. However, these patients werenotprotected frommeningococcal disease after immunization.
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