Identification of Corynebacterium jeikeium and Corynebacterium CDC group D2 with the API 20 Strep system.
ABSTRACT A total of 170 strains of Corynebacterium jeikeium and 23 strains of Corynebacterium group D2 were examined in three British laboratories using the API 20 Strep identification system and three supplementary tests (catalase production, urease production and nitrate reduction). The isolates were collected from clinical specimens in various laboratories over a three-year period. The two species produced consistent reactions in these tests after 24 h. Two tests were highly discriminatory, with positive reactions for ribose fermentation seen for Corynebacterium jeikeium while urease production was observed with Corynebacterium group D2. This method allows routine clinical laboratories to rapidly identify these emerging pathogens.
- Clinical Microbiology Newsletter 01/1995; 17(23):177-180.
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ABSTRACT: To identify rapidly and accurately coryneform bacteria, using a commercial strip system. Ninety eight strains of Corynebacterium species and 62 additional strains belonging to genera Erysipelothrix, Oerskovia, Rhodococcus, Actinomyces, Archanobacterium, Gardnerella and Listeria were studied. Bacteria were identified using conventional biochemical tests and a commercial system (API-Coryne, BioMèrieux, France). Fresh rabbit serum was added to fermentation tubes for Gardnerella vaginalis isolates. One hundred and five out of the 160 (65.7%) organisms studied were correctly and completely identified by the API Coryne system. Thirty five (21.8%) more were correctly identified with additional tests. Seventeen (10.6%) organisms were not identified by the system and three (1.9%) were misidentified. The system was a good alternative for identification of coryneform organisms. When occasionally performed with some additional tests, this method permits reliable and rapid identification of coryneform organisms compared with conventional methods.Journal of Clinical Pathology 09/1994; 47(8):756-9. · 2.44 Impact Factor
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ABSTRACT: The Rapid CORYNE system for identification of aerobic, nonsporeforming, gram-positive rods was evaluated according to the manufacturer's instructions with 177 organisms. After inoculation with a heavy suspension of growth, strips containing 20 cupules were incubated for 24 h, reagents were added, and the results of 21 biochemical reactions were recorded as numerical profiles. The strains consisted of pathogenic species of the genus Corynebacterium, primarily C. diphtheriae (n = 29), opportunistic species of Corynebacterium including C. jeikeium (n = 75), recognized species of non-corynebacteria such as Gardnerella and Arcanobacterium (n = 51), and Centers for Disease Control (CDC) coryneform groups (n = 22). Results from single tests read after 24 h yielded correct identifications to species level with no additional tests for 26 (89.7%) of the pathogenic species; 64 (85.3%) of the opportunistic organisms; 51 (100%) of the non-corynebacteria, and 8 (36.4%) of the CDC coryneform groups. Supplemental tests produced the correct identification for three additional pathogenic isolates (100% total) and four additional isolates from the opportunistic species (90.6% total). Twelve of the 15 isolates not identified by the system were in the CDC coryneform groups. Four of the six misidentified and one of the unidentified isolates were C. matruchotii, which was not included in the data base. The system is an excellent rapid alternative to conventional biochemical tests.Journal of Clinical Microbiology 08/1992; 30(7):1692-5. · 4.07 Impact Factor