Article

Prostaglandin secretion by perifused porcine endometrium: Further evidence for an endocrine versus exocrine secretion of prostaglandins

Department of Dairy Science, University of Florida, Gainesville 32611.
Prostaglandins 04/1988; 35(3):327-41. DOI: 10.1016/0090-6980(88)90126-8
Source: PubMed

ABSTRACT Bilateral perifusion devices were utilized for measurement of prostaglandin secretion by luminal and myometrial surfaces of porcine endometrium. Tissues were collected from Days 10, 12 and 14 pregnant, Day 14 cyclic and Day 14 estrogen-induced pseudopregnant gilts. Each tissue was placed into duplicate perifusion devices and perifused with Krebs-Ringer Bicarbonate solution at 3 ml/10 min for 2 h, fractions collected every 10 min and oxytocin (1 IU/ml) perifused during fractions 6-10 to the luminal side of one chamber and to the myometrial side of the other chamber. Secretion rates of PGF were higher (P less than 0.05) than PGE2 for each status. Secretion rates of PGF and PGE2 were higher (P less than 0.01) from the luminal side for Day 12 pregnant, Day 14 pregnant and Day 14 pseudo-pregnant gilts, whereas secretion was higher from the myometrial side for Day 10 pregnant and Day 14 cyclic gilts. Oxytocin increased (P less than 0.01) prostaglandin secretion from the luminal side regardless of reproductive status. Pregnancy at Day 12 and Day 14, as well as estrogen treatment, were associated with prostaglandin secretion in a luminal (exocrine) orientation versus a myometrial (endocrine) orientation for Day 14 cyclic and Day 10 pregnant gilts. These data indicate an estrogen associated switch between Days 10 and 12 of pregnancy from an endocrine to an exocrine secretion of prostaglandins.

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    • "The concept of a servomechanism in pigs is based on evidence that E2 induces PRLR in uterine LE and GE for shifting secretion of PGF from an endocrine to an exocrine direction for pregnancy recognition [80]. As well, there are synergies between P4, E2 and PRL from the maternal anterior pituitary gland that allow for greater secretory activity of uterine GE in pigs [57-60]. "
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    • "In addition, treatment with oestradiol enhanced the response of luminal epithelial cells to OT and increased PGF 2α secretion from the apical surface . These latter effects are in general agreement with the increased responsiveness to OT of luminal epithelial cells from pregnant pigs (Uzumcu et al. 2000) and are likely to contribute to the increased exocrine secretion of PGF 2α in response to OT that occurs during early pregnancy (Gross et al. 1988; Carnahan et al. 1999). In contrast, oestradiol clearly reduced the PGF 2α secretory response of stromal cells to OT on Day 16 in the present study, an effect that could contribute to reduced endocrine secretion of PGF 2α from cells that are in closest proximity to the uterine vasculature during pregnancy recognition (Carnahan et al. 1996; Edgerton et al. 1996). "
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    ABSTRACT: These studies were undertaken to determine how treatment with 100 nM progesterone and/or 10 nM oestradiol-17beta acutely (3 h; Experiment 1) or chronically (72 h; Experiments 2-4) influenced basal and oxytocin (OT)-stimulated prostaglandin (PG) F(2alpha) secretion, in enriched cultures of pig endometrial luminal epithelial, glandular epithelial and stromal cells obtained on Day 16 (Experiments 1, 2 and 4) or Day 12 (Experiment 3) after oestrus. In Experiment 1, acute treatment with progesterone stimulated PGF(2alpha) secretion from each cell type on Day 16, whereas acute oestradiol treatment inhibited the stimulatory action of progesterone on PGF(2alpha) secretion only in glandular epithelial cells. In Experiment 2, OT stimulated phospholipase (PL) C activity in luminal epithelial cells on Day 16 only in the presence of chronic oestradiol treatment. For glandular epithelial cells on Day 16, OT stimulated PLC activity only in the presence of chronic treatment with steroid. In stromal cells on Day 16, OT stimulated PLC activity in the absence of steroids and the response to OT was further enhanced by oestradiol. In the absence of chronic treatment with steroid, OT did not stimulate PGF(2alpha) secretion from luminal epithelial cells, but oestradiol induced a response to OT. For glandular epithelial cells, OT-induced PGF(2alpha) secretion was not altered by steroids, whereas the stimulatory response to OT was inhibited by oestradiol or progesterone in stromal cells. For endometrial cells obtained on Day 12 after oestrus in Experiment 3, OT only stimulated PGF(2alpha) release from glandular epithelial and stromal cells. For luminal epithelial cells obtained on Day 16 after oestrus and cultured under polarizing conditions in Experiment 4, secretion of PGF(2alpha) occurred preferentially from the basolateral surface and was stimulated by OT more from the basolateral surface than from the apical surface. Oxytocin-induced PGF(2alpha) secretion from the apical surface was enhanced by chronic treatment with oestradiol, whereas that from the basolateral surface was enhanced by chronic treatment with progesterone. In summary, oestradiol enhanced OT-induced PGF(2alpha) secretion from the apical surface of luminal epithelial cells and reduced the response of stromal cells to OT, actions that may contribute to the reorientation of PGF(2alpha) from endocrine secretion (i.e. towards the uterine vasculature) to exocrine secretion (i.e. towards the uterine lumen) during pregnancy recognition in pigs.
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    • "In addition, treatment with oestradiol enhanced the response of luminal epithelial cells to OT and increased PGF 2α secretion from the apical surface . These latter effects are in general agreement with the increased responsiveness to OT of luminal epithelial cells from pregnant pigs (Uzumcu et al. 2000) and are likely to contribute to the increased exocrine secretion of PGF 2α in response to OT that occurs during early pregnancy (Gross et al. 1988; Carnahan et al. 1999). In contrast, oestradiol clearly reduced the PGF 2α secretory response of stromal cells to OT on Day 16 in the present study, an effect that could contribute to reduced endocrine secretion of PGF 2α from cells that are in closest proximity to the uterine vasculature during pregnancy recognition (Carnahan et al. 1996; Edgerton et al. 1996). "
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