Article

Immobilized pH gradient isoelectric focusing of cerebrospinal fluid proteins.

Department of Neurology, University of Tampere, Sweden.
Electrophoresis (impact factor: 3.3). 10/1988; 9(9):582-8. DOI:10.1002/elps.1150090920 pp.582-8
Source: PubMed

ABSTRACT Isoelectric focusing in immobilized pH gradients, supplemented with 0.5% w/v carrier ampholytes was applied for studies of native proteins, especially immunoglobulin G, in cerebrospinal fluid and serum. All 72 paired samples were run on pH 4-10 gels; 25 of them were also examined in pH 7-10 gels. Silver staining and nitrocellulose blotting with amplified immunoperoxidase detection of immunoglobulin G were used for protein visualization. Intrathecally produced immunoglobulin G was resolved into sharply focused, straight and easily identifiable fractions. The pH gradients were stable and the inter-gel reproducibilities of individual immunoglobulin G patterns were good.

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Keywords

0.5% w/v carrier ampholytes
 
72 paired samples
 
amplified immunoperoxidase detection
 
cerebrospinal fluid
 
immobilized pH gradients
 
immunoglobulin G
 
individual immunoglobulin G patterns
 
inter-gel reproducibilities
 
native proteins
 
pH gradients
 
Silver staining
 

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