Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA class I and III regions by field inversion gel electrophoresis.

Medizinische Klinik, University of Tübingen, Federal Republic of Germany.
Immunogenetics (Impact Factor: 2.49). 02/1988; 27(1):66-9. DOI: 10.1007/BF00404447
Source: PubMed


Available from: Elisabeth H Weiss, May 14, 2015
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    ABSTRACT: The restriction fragment length polymorphism of the human tumour necrosis factor (TNFα) region was investigated by means of 20 different restriction enzymes and a human TNFα cDNA probe. Only one of the enzymes, NcoI, revealed a polymorphic pattern consisting of fragments of 10.5 and 5.5 kb, which behaved as alleles. In a panel of 108 random, healthy, unrelated Danes, the phenotype frequencies of the 10.5 and 5.5 kb bands were 0.94 and 0.53 and the gene frequencies were 0.71 and 0.29, respectively. The test for Hardy-Weinberg equilibrium showed no significant deviation from the expected values. The 5.5 kb band was strongly positively associated with HLA-DR3, HLA-B8, and HLA-A1. In 22 patients with primary biliary cirrhosis a significantly (corrected P= 0.024) decreased frequency of the 10.5 kb fragment was found. Additional studies are in progress to substantiate this association.
    Scandinavian Journal of Immunology 08/1989; 30(2):185-189. DOI:10.1111/j.1365-3083.1989.tb01200.x · 1.88 Impact Factor
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    ABSTRACT: We studied the effect of the different types of interferons on the production of cytotoxin by human peripheral blood mononuclear cells (PBMCs) stimulated with the mitogen phytohemagglutinin (PHA). Maximum secreted levels of cytotoxin were observed at day 3 in cul- ture and consisted of both tumor necrosis factor � (TNF- a) and lymphotoxin as determined by specifIc antibodies. Type I interferons (IFN-a and IFN-f3) consistently sup- pressed cytotoxin production. Both TNF-a and lym- photoxin were significantly suppressed. Mean suppres- sion by IFN-a and IFN-f3 (1000 U/mi) was 56 and 66%, respectively, in PBMCs from 18 different donors. The suppressive effects of IFN-a and IFN-f.3 on cytotoxin production were dose responsive over a range of 10 to 1000 U/mi. Type II interferon (IFN-'y) did not have con- sistent significant effects. Pretreatment with IFN-a or IFN-/3 for 24 or 48 h prior to PHA stimulation also resulted in significant suppression. Supplementation with interleukin-2 (10 U/ml) or IFN-'y (1000 U/mi) did not overcome cytotoxin suppression by IFN-a or IFN-f3. Cytotoxin suppression by IFN-a and IFN-f3 together ap- peared to be noninteractive. Suppression appeared not to � be due to blockade of the cytotoxin release, since both cell-associated cytotoxin and secreted cytotoxin were sup- pressed to the same level. These results demonstrated that cytotoxin and lymphotoxin production by PHA- stimulated PBMCs could be down-regulated by type I in- terferons and that there is a substantial difference be- tween the action of type I interferons and type II interfe- rons (IFN-y) in modulating the biosynthesis of cytotoxins.J. Lcukoc. Biol. 52: 165-172; 1992.
  • European Journal Of Haematology 09/2009; 43(3):255-256. DOI:10.1111/j.1600-0609.1989.tb00292.x · 2.41 Impact Factor