Stewart, C. & Burke, B. Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B. Cell 51, 383-392
Harvard University, Cambridge, Massachusetts, United StatesCell (Impact Factor: 32.24). 12/1987; 51(3):383-92. DOI: 10.1016/0092-8674(87)90634-9
The nuclear lamina in adult mammalian somatic cells is composed of three major proteins, lamins A, B, and C. The expression of these proteins during the differentiation of teratocarcinomas and mouse embryogenesis is described. Embryos up to day 8 of gestation and embryonal carcinoma (EC) cells express only a single lamin species closely resembling, if not identical to, lamin B. Lamins A and/or C were detected in fertilized eggs, but disappear during the first 2-4 cleavage divisions, only reappearing in 8 day post-implantation embryos. These two lamins are absent from EC cells, but are strongly expressed in some of their derivatives. These results show that cells of the early mouse embryo do not have a functional requirement for lamins A and C and imply that the structural organization of the nucleus may change fundamentally during embryogenesis.
The Journal of Cell Biology 11/2015; 211(3):533-551. DOI:10.1083/jcb.201504035 · 9.83 Impact Factor
- "In vertebrates, lamin proteins have been divided into A and B types, based on sequence homologies. Whereas B-type lamins are ubiquitously expressed, A-type lamins, such as lamin A and C (hereafter lamin A/C), are developmentally regulated, being absent in the early embryo and expressed in differentiating cells (Stewart and Burke, 1987; Röber et al., 1989), suggesting a role in cell differentiation (Lanzuolo, 2012; Collas et al., 2014). Indeed, beyond providing mechanical support to the nucleus, lamins are involved in the regulation of gene expression at various levels (Shumaker et al., 2006; Scaffidi and Misteli, 2008; Méjat et al., 2009; Lund et al., 2013; McCord et al., 2013). "
[Show abstract] [Hide abstract]
- "Supplemental Material can be found at: 2006; Eckersley-Maslin et al., 2013). LB1 continues to be expressed in all cell types throughout development while LA/C and LB2 expression varies across tissues (Stewart and Burke, 1987; Rober et al., 1989). A large number of mutations in the LMNA gene causing a wide range of genetic disorders, collectively called laminopathies, often cause misshapen nuclei accompanied by significant changes in chromatin organization (Shimi et al., 2008). "
ABSTRACT: The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina remains poorly defined. Using three-dimensional Structured Illumination Microscopy and computational image analysis, we have characterized the supramolecular structures of lamin A, C, B1 and B2 in mouse embryo fibroblast nuclei. Each isoform forms a distinct fiber meshwork, having comparable physical characteristics with respect to mesh edge length, mesh face area and shape, and edge connectivity to form faces. Some differences were found in face areas between isoforms due to variation in the edge lengths and number of edges per face, suggesting that each meshwork has somewhat unique assembly characteristics. In fibroblasts null for the expression of either lamins A/C or lamin B1, the remaining lamin meshworks are altered compared with the lamin meshworks in wild type nuclei or nuclei lacking lamin B2. Nuclei lacking LA/C exhibit slightly enlarged meshwork faces and some shape changes, whereas LB1-deficient nuclei exhibit primarily a substantial increase in face area. These studies demonstrate that individual lamin isoforms assemble into complex networks within the nuclear lamina and that A-type and B-type lamins have distinct roles in maintaining the organization of the nuclear lamina.Molecular biology of the cell 08/2015; 26(22). DOI:10.1091/mbc.E15-07-0461 · 4.47 Impact Factor
[Show abstract] [Hide abstract]
- "Immunofluorescence staining and immunoblotting with isotype specific anti-Lamin antibodies in mouse embryos show low expression of Lamin A/C in ESCs, which increases as cells differentiate (Constantinescu et al., 2006; Eckersley-Maslin et al., 2013). In mice, the increase in Lamin A/C expression is initiated as early as embryonic day 9 and as late as in the adult animal depending on the tissue type (Stewart and Burke, 1987; Rober et al., 1989). In direct support of a role for Lamin A in cell differentiation, experiments in mouse cells testing the effect of Lamin A levels on somatic to iPS cell reprogramming show that depletion of Lamin A accelerates the transition to pluripotency, while cells overexpressing Lamin A take longer to reprogram (Zuo et al., 2012). "
ABSTRACT: The eukaryotic cell nucleus houses an organism's genome and is the location within the cell where all signaling induced and development-driven gene expression programs are ultimately specified. The genome is enclosed and separated from the cytoplasm by the nuclear envelope (NE), a double-lipid membrane bilayer, which contains a large variety of trans-membrane and associated protein complexes. In recent years, research regarding multiple aspects of the cell nucleus points to a highly dynamic and coordinated concert of efforts between chromatin and the NE in regulation of gene expression. Details of how this concert is orchestrated and how it directs cell differentiation and disease are coming to light at a rapid pace. Here we review existing and emerging concepts of how interactions between the genome and the NE may contribute to tissue specific gene expression programs to determine cell fate.Frontiers in Genetics 03/2015; 6:95. DOI:10.3389/fgene.2015.00095
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.