Carbon-13 NMR studies of chloroplast membranes: carbon-13 enrichment without 13C-13C couplings.

Biology Department, Carleton University, Ottawa, Canada.
Journal of Biochemical and Biophysical Methods (Impact Factor: 1.81). 01/1988; 15(3-4):229-31. DOI: 10.1016/0165-022X(87)90121-7
Source: PubMed

ABSTRACT A novel approach to carbon-13 (13C) enrichment of chloroplast membranes (and plant materials in general) is presented for 13C-nuclear magnetic resonance (13C-NMR) studies. The method minimizes the occurrence of spectral complications arising from 13C-13C couplings resulting from a statistical distribution of 13C within the molecule with low probability of encountering two 13C atoms adjacent to each other. This is achieved by growing the plants in light surrounded by an atmosphere containing 1/3rd 12CO2 and 2/3rd 13CO2, liberated by weak acid-treatment of a mixture of corresponding barium carbonate salts.

  • Source
    Indian journal of biochemistry & biophysics 01/1987; 24(6):177-178.. · 1.08 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Spinach chloroplast membranes and aqueous dispersions of their extracted lipids have been studied by spin label (stearic acid) electron spin resonance and carbon-13 nuclear magnetic resonance techniques. Combined with electron microscope studies, first systematic evidence is found for the existence of a dynamic lipid-bilayer structure in the chloroplast membranes.
    Journal of Biosciences 12/1990; 15(4):281-288. DOI:10.1007/BF02702669 · 1.94 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Aqueous dispersions of lipids isolated from spinach chloroplast membranes were studied by electron microscopy after negative staining with phosphotungstic acid. Influence of low temperature (5°C for 24 h) was also investigated. It was observed that when contacted with water, these lipids, as such, formed multilamellar structures. Upon sonication, these multilamellar structures gave rise to a clear suspension of unilamellar vesicles varying in size (diameter) between 250 and 750 Å. When samples of sonicated unilamellar vesicles were stored at 5°C for 24 h or more, they revealed a variety of lipid aggregates including liposomes, cylindrical rods (about 100 Å wide and up to 3600 Å long), and spherical micellar structures (100-200 Å in diameter)—thus indicating phase separation of lipids.
    Journal of Biosciences 07/1990; 15(2):93-98. DOI:10.1007/BF02703373 · 1.94 Impact Factor