The lysozyme enhancer: Cell-specific activation of the chicken lysozyme gene by a far upstream DNA element

The EMBO Journal (Impact Factor: 10.43). 05/1986; 5(4):719-24.
Source: PubMed


The chicken lysozyme gene is constitutively expressed in macrophages and controlled by steroid hormones in the oviduct. We have investigated the influence of the 5' noncoding region of this gene on its cell-specific transcriptional activation. In transient transfection experiments we have identified a far-upstream cell-specific enhancer element 6.1 kb 5' to the transcriptional start site of the lysozyme gene. Transcription from the lysozyme gene promoter is induced by this element in a position- and orientation-independent manner in lysozyme-producing myeloid cells (HBCI), but not in non-producing chicken embryo fibroblasts (CEF-38). The enhancer region correlates with a DNase-hypersensitive chromatin site which is only detectable in cells of tissues in which the lysozyme gene is transcribed. We suggest that this far-upstream element is involved in the tissue-specific control of lysozyme gene activity.

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Available from: Manfred Theisen,
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    • "Lee) Tel.: +82-42-821-5779 Fax: +82-42-825-9754 E-mail: 2010년 10월 5일 투고 2010년 10월 29일 심사완료 2010년 12월 13일 게재확정 Theisen et al., 1986 "
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    ABSTRACT: Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.
    01/2010; 37(3).
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    • "Other HSP70/CAT plasmids contain the promoter region of the human HSP70 gene (Kaddurah-Daouk et al. 1987). pLYSCAT constructs contain the promoter region of the chicken lysozyme gene (Theisen et al. 1986). (534) A sequence to which v-myb protein binds in vitro (Biedenkapp et al. 1988). "
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    ABSTRACT: The retroviral oncogene v-myb encodes a nuclear, sequence-specific DNA-binding protein. To investigate the possibility that v-myb encodes a transcriptional regulator, we used a transient cotransfection assay to explore the potential of v-myb to influence the expression of other genes. We found that expression of a chicken lysozyme promoter/CAT gene construct was activated by v-myb in the presence of myb-specific binding sites. Action was not observed with a truncated v-myb protein lacking its DNA-binding domain. We also observed that expression of a hybrid human HSP70 promoter/CAT gene, lacking myb-specific binding sites, was activated by v-myb. However, in this case, the truncated v-myb protein, which lacked its DNA-binding domain, also activated HSP70/CAT expression, indicating that trans-activation of this gene construct was independent of the sequence-specific DNA-binding activity of the v-myb protein. These observations suggest that v-myb encodes a trans-activator and that activation of gene expression occurs by two different mechanisms, one of which involves specific binding of v-myb protein to the regulated gene.
    Genes & Development 11/1989; 3(10). DOI:10.1101/gad.3.10.1582 · 10.80 Impact Factor
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    • "A dominant gene for high lysozyme activity has been detected in cattle (Lie & Solbu 1983). Moreover, a corresponding low-level recessive gene has been found in rabbits (Prieur & Camara 1979), and positive and negative regulatory genes, i.e. enhancers and silencers, of chicken lysozyme have been isolated, cloned and their expression studied by transfection experiments in chicken cell lines (Theisen et al. 1986, Baniahmad et al. 1987, Steiner et al. 1987). Several studies indicate that lysozyme may play a role in fish a s a defence mechanism against infectious disease (Fange et al. 1976, Murray & Fletcher 1976, Lundblad et al. 1979, Lindsay 1986). "

    Diseases of Aquatic Organisms 02/1989; 6(1):1-5. DOI:10.3354/dao006001 · 1.75 Impact Factor
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