Modulation of the natural killer cell activity in pregnant mice alters the spontaneous abortion rate.
ABSTRACT Effector cells associated with an aborting fetus appear to be both thymus derived (T) and natural killer (NK) cells. In order to test the hypothesis that NK cells are a major effector mediating early spontaneous abortion (less than day 8-10), CBA female mice mated by DBA/2 males were treated with either polyinosinic/cytidylic acid (poly I:C) to boost NK activity, or rabbit anti-asialo GM1 (RaASGM1) to decrease NK activity. The results of the NK assays of the spleens of treated mice confirmed that the reagents had the expected effect on NK activity and an inspection of the uteri indicated a significant increase in aborted embryos after poly I:C and a marked decrease in spontaneous abortions after RaASGM1 treatment. Therefore, spontaneous abortions may be mediated in part by the cytotoxic activity of unregulated NK cells.
- [Show abstract] [Hide abstract]
ABSTRACT: This review is an example of the use of an animal model to try to understand the immune biology of pregnancy. A well-known model of recurrent spontaneous pregnancy loss is put in clinical, historical and theoretical context, with emphasis on T cell biology.Reproduction 01/2014; · 3.56 Impact Factor
Article: τ EDM at low energies[Show abstract] [Hide abstract]
ABSTRACT: Low energy tau pair production, at B factories and on top of the T resonances, allows for a detailed investigation on the CP violation at the electromagnetic tau pair production vertex. High statistic available at low energies offers the opportunity for an independent analysis of CP-violation in the τ lepton physics. We show that stringent and independent bounds on the T electric dipole moment, competitive with the high energy measurements, can be established in low energies experiments.Nuclear Physics B - Proceedings Supplements 01/2003; 123:69-73. · 0.88 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Shimada S, Iwabuchi K, Watano K, Shimizu H, Yamada H, Minakami H, Onoé K. Expression of allograft inflammatory factor-1 in mouse uterus and poly(I:C)-induced fetal resorption. AJRI 2003; 50:104–112 © Blackwell Munksgaard, 2003 Problem: To investigate whether the allograft inflammatory factor-1 (AIF-1) is expressed and plays a role in the reproductive system. Method of study: AIF-1 expression was examined in uteri of non-pregnant and pregnant mice by Northern blot analysis, RT-PCR and immunohistochemistry. Results: The expression of AIF-1 varied during the estrous cycle with a peak at estrus. After the insemination, the expression of AIF-1 mRNA diminished gradually and again increased in the pre-implantation or implantation period in allogeneic or syngeneic pregnancy, respectively. Enhanced expressions of AIF-1, tumor necrosis factor-α (TNF-α) and nitric oxide synthase 2 (NOS2) mRNA were observed in the embryos of resorption-prone pregnancy injected with poly(I:C). Conclusions: This study demonstrated for the first time that AIF-1 was expressed in uterus. The expression level was associated with the population size of macrophage and varied during the estrous cycle and the pregnancy period. The augmented expression of AIF-1 with concomitant expressions of TNF-α and NOS2 mRNA in poly(I:C)-injected mice suggests a correlation between AIF-1 production and fetal resorption.American Journal Of Reproductive Immunology 01/2003; 50(1):104-112. · 3.32 Impact Factor