Production of leukemic blast growth factor by a human bladder carcinoma cell line

Blood (Impact Factor: 10.45). 10/1985; 66(3):748-51.
Source: PubMed


Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.

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    • "T cells were then purified by a negative selection method using saturating amounts of CD11b, CD21 and CD68 antibodies (DAKO, San Diego, CA, USA), followed by elimination using anti-mouse IgG goat antibody-coated Dynabeads (Dynal, Oslo, Norway). The HTB-9 cell line, established from a human urinary bladder carcinoma and known to produce abundant amounts of G-CSF (Hoang & McCulloch, 1985), was generously provided by the Kirin Corporation (Tokyo, "
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    ABSTRACT: We previously demonstrated that, in about 30% of primary adult T-cell leukaemia (ATL) cases, the leukaemic cells proliferated in response to granulocyte colony-stimulating factor (G-CSF). In the present report, we describe five patients with the acute leukaemia type of ATL who showed marked neutrophilia and elevated serum G-CSF concentrations in the absence of infection. We further examined two of these patients for detailed clinical features and cellular characteristics of the tumour cells. The white blood cell counts of both patients were 62 x 10(9)/l, consisting of approximately 90% neutrophils and 10% ATL cells. Serum concentrations of G-CSF in the two patients were 138 pg/ml and 93 pg/ml. The G-CSF concentrations in supernatants of short-term cultures of the patients' peripheral blood T-cells were 2 5 pg/ml and 13 pg/ml respectively. Immunostaining with anti-G-CSF antibody demonstrated G-CSF production by primary ATL cells in both cases. The neutrophil count fluctuated simultaneously with activity of ATL. Primary ATL cells from one patient were shown to proliferate in response to G-CSF in vitro. These results suggest autocrine growth stimulation of primary ATL cells in a subgroup of patients.
    British Journal of Haematology 11/2000; 111(1):208-15. DOI:10.1046/j.1365-2141.2000.02257.x · 4.71 Impact Factor
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    ABSTRACT: The blast cells of acute myeloblastic leukemia (AML) may be considered as a renewal population, maintained by blast stem cells capable of both self-renewal and the generation of progeny with reduced or absent proliferative potential. Blast progenitor renewal is manifested in suspension culture by an exponential increase in clonogenic cells. This growth requires that two conditions be met: first, the cultures must contain growth factors in media conditioned either by phytohemagglutinin (PHA)-stimulated mononuclear leukocytes (PHA-LCM), or by cells of the continuous bladder carcinoma line HTB9 (HTB9-CM). Second, the cell density must be maintained at 10(6) blasts/ml; this may be achieved by adding irradiated cells to smaller numbers of intact blasts. We are concerned with the mechanism of the feeding function. We present evidence that (a) cell-cell contact is required. (b) Blasts are heterogeneous in respect to their capacity to support growth. (c) Fractions containing membranes from blast cells will substitute for intact cells in promoting the generation of new blast progenitors in culture. (d) This membrane function may be specific for AML blasts, since membranes from blasts of lymphoblastic leukemia or normal marrow cells were inactive.
    Journal of Experimental Medicine 12/1985; 162(5):1435-43. DOI:10.1084/jem.162.5.1435 · 12.52 Impact Factor
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    ABSTRACT: The ability of conditioned media from the 5637 cell line and human placenta (HPCM) to stimulate the in vitro growth of human early erythroid and mixed myeloid/erythroid clones was tested and compared to phytohemagglutinin-stimulated leukocyte supernatants (PHA-LCM). Both 5637 supernatant and PHA-LCM were equally effective with a linear dose-response relationship. HPCM at various concentrations did not exhibit burst-promoting activity (BPA). Thus, "pluripoietin"-containing media provide a large-scale source of BPA similar in its biological activity to standard sources used for studying human hematopoiesis in vitro.
    International journal of cell cloning 01/1986; 4(6):424-31. DOI:10.1002/stem.5530040604
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