Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.

Nature (Impact Factor: 42.35). 01/1985; 313(6001):358-63. DOI: 10.1038/313358a0
Source: PubMed

ABSTRACT Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose 1,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: -Plant secondary metabolites are economically important, in such different fields as drugs, fragrances, pigments, food additives and pesticides. An alternative to their extraction from the plant, is to synthesize them from in vitro cell cultures. After a brief revision of the historical background of the in vitro production, its technical aspects according to its diverse forms. along with a number of applications including industrial-scale products. illustrate this process. Economic data, with the indication of limiting factors and an analysis of potential valuable developments, complement the assessment of this new process. The market share, the investment-profit ratio, the competition with other applications will also be valuable data for decision-making.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.
    Pesquisa Agropecuária Brasileira 01/2013; 48(1):73-79. DOI:10.1590/S0100-204X2013000100010 · 0.68 Impact Factor
  • Source