Sterol metabolism. XXX. Cholesterol 25-hydroperoxide metabolism.
ABSTRACT The metabolism of cholesterol 25-hydroperoxide by subcellular fractions of rat and calf liver and of rat kidney was examined. The sole metabolic transformation observed was the reduction of the 25-hydroperoxide to the corresponding 25-alcohol 5-cholestene-3β,25-diol. No evidence of enzymic rearrangement of the 25-hydroperoxide to vicinal diol products was adduced. Neither cholesterol 25-hydroperoxide nor 5-cholestene-3β,25-diol was converted by rat liver homogenates to bile acids. Attempts to demonstrate cholesterol 25-hydroperoxide as a putative intermediate in the biosynthesis of bile acids from cholesterol in rat liver homogenates and calf liver microsomes by trapping techniques were unsuccessful. It was concluded that cholesterol 25-hydroperoxide is not implicated in cholesterol metabolism in liver or kidney tissues.
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ABSTRACT: We make a bibliographical review on the main aspects related to the cholesterol oxidation products, including their biological effects, their presence and formation in food and their analytical determination.Se realiza una revisión bibliográfica de los aspectos nutricionales y bromatológicos de los llamados oxiesteroles. Se recogen los efectos biológicos descritos para estos compuestos, su presencia y formación en alimentos y la metodología analítica para su determinación.Grasas y Aceites 01/1991; DOI:10.3989/gya.1991.v42.i1.1280 · 1.08 Impact Factor
- 01/2004; 20. DOI:10.6018/j/17731
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ABSTRACT: In order to rationalize multiphasic dose-response data evincing mutagenicity towards Salmonella typhimurium TA1537 for sterol hydroperoxides 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide and 3 beta-hydroxycholest-5-ene-7 alpha-hydroperoxide their metabolism by the bacterial test strain was investigated. The 5 alpha-hydroperoxide was isomerized to the 7 alpha-hydroperoxide and reduced to 5 alpha-cholest-6-ene-3 beta,5-diol; the 7 alpha-hydroperoxide was reduced to cholest-5-ene-3 beta,7 alpha-diol and transformed to 3 beta-hydroxycholest-5-en-7-one. The 3 beta,5 alpha-diol and 3 beta,7 alpha-diol were not interconverted nor was either transformed to the 7-ketone.Journal of Steroid Biochemistry 03/1987; 26(2):259-64. DOI:10.1016/0022-4731(87)90080-X