Demonstration of type-specific influenza antibody in mammalian and avian sera by immunodiffusion.

Bulletin of the World Health Organisation (Impact Factor: 5.25). 02/1970; 42(5):779-85.
Source: PubMed

ABSTRACT The detection of antibody against the ribonucleoprotein antigen of influenza virus is useful because its type-specificity allows the use of serological surveys to detect evidence of recent infections. Antigenic differences between strains limit the usefulness of the techniques, such as the haemagglutination-inhibition test, that detect antibody against surface antigens.This paper describes an agar-gel precipitation (AGP) test that will detect type-specific antibody in avian or mammalian sera. Convalescent levels of antibody against either type A or B influenza virus were demonstrated in human sera. Positive but inconsistent results were obtained with swine sera. The antigens used in the AGP test are non-infectious and stable. The test is easy and economical to perform. Its sensitivity compares favourably with that of the complement-fixation test using human and equine sera.While not a replacement for any of the serological tests at present in current use, the AGP test should prove useful in a variety of diagnostic and research situations.

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    ABSTRACT: Cottontails (Sylvilagus spp.) are common mammals throughout much of the U.S. and are often found in peridomestic settings, potentially interacting with livestock and poultry operations. If these animals are susceptible to avian influenza virus (AIV) infections and shed the virus in sufficient quantities they may pose a risk for movement of avian influenza viruses between wildlife and domestic animals in certain situations.
    PLoS ONE 08/2014; 9(8):e102513. · 3.53 Impact Factor


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