Article

Contamination of hospital disinfectants with Pseudomonas species

British medical journal 05/1967; 2(5545):153-5. DOI: 10.1136/bmj.2.5545.153
Source: PubMed
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    • "Once a biofilm has established on a surface, cleaning and eradicating the biofilm from that surface becomes a challenge, as the dispenser remediation experiments demonstrated. The ineffectiveness of washing soap bottles dates back to the 1960s, so these findings are not surprising (Burdon and Whitby 1967). "
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    ABSTRACT: Recent studies evaluating bulk soap in public restroom soap dispensers have demonstrated up to 25% of open refillable bulk-soap dispensers were contaminated with ~ 6 log(10)(CFU ml(-1)) heterotrophic bacteria. In this study, plastic counter-mounted, plastic wall-mounted and stainless steel wall-mounted dispensers were analyzed for suspended and biofilm bacteria using total cell and viable plate counts. Independent of dispenser type or construction material, the bulk soap was contaminated with 4-7 log(10)(CFU ml(-1)) bacteria, while 4-6 log(10)(CFU cm(-2)) biofilm bacteria were isolated from the inside surfaces of the dispensers (n = 6). Dispenser remediation studies, including a 10 min soak with 5000 mg l(-1) sodium hypochlorite, were then conducted to determine the efficacy of cleaning and disinfectant procedures against established biofilms. The testing showed that contamination of the bulk soap returned to pre-test levels within 7-14 days. These results demonstrate biofilm is present in contaminated bulk-soap dispensers and remediation studies to clean and sanitize the dispensers are temporary.
    Biofouling 01/2012; 28(1):99-109. DOI:10.1080/08927014.2011.653637 · 3.70 Impact Factor
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    Journal of the Society of Cosmetic Chemists 01/1972; 23.
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