Article

Enzymatic synthesis of flavin adenine dinucleotide.

Applied Biochemistry and Biotechnology (impact factor: 1.94). 07/1983; 8(3):237-47. pp.237-47
Source: PubMed

ABSTRACT A new and simple enzymatic method for the synthesis of flavin adenine dinucleotide (FAD) from flavin mononucleotide by the transadenylylation reaction using microbial cells is described. Among various microorganisms tested, Artherobacter globiformis IFO 12138 and two soil bacteria were selected as useful enzyme sources. Under suitable reaction conditions, the amount of FAD synthesized was 2.25 mumol/mL with cells of A. globiformis. The transadenylylation reaction could be coupled with the ATP supplying system through a glycolysis process with yeast.

0 0
 · 
0 Bookmarks
 · 
16 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

Artherobacter globiformis IFO 12138
 
ATP
 
FAD synthesized
 
glycolysis process
 
microbial cells
 
simple enzymatic method
 
soil bacteria
 
suitable reaction conditions
 
transadenylylation reaction
 
useful enzyme sources
 
yeast
 

S Shimizu