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    ABSTRACT: In this study we have identified and characterized an expanded granular lymphocyte population in a patient with anemia and granulocytopenia. Granular lymphocytes were identified through the presence of cytoplasmic azurophilic granules, the dispersed granular pattern of cytochemical staining for acid hydrolases, and the ultrastructural localization of acid phosphatase within the granules. The surface phenotype of the granular lymphocytes was E+, FcR-, Leu 4+, Leu 2+, D 12+ (Leu 15+), OKM1+, and Leu 7+. This phenotype has not been reported previously in patients with similar features. Functional studies on FACS-purified populations showed that the patient's granular lymphocytes responded poorly to T-cell mitogens and were inefficient in NK and ADCC assays but exerted a potent suppressor effect on both T-cell proliferation and B-cell differentiation. The phenotype and functions of the expanded granular lymphocyte population correspond to those of a subset of normal E rosette-forming granular lymphocytes.
    Journal of Clinical Immunology 08/1984; 4(4):326-34. DOI:10.1007/BF00915301 · 3.18 Impact Factor
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    ABSTRACT: It has recently become clear that in the spleen and blood of both rodents and man that a unique subpopulation of lymphocyte is the mediator of virtually all of the inherent natural killer (NK) and antibody-dependent cell-mediated cytotoxic (ADCC) activity. Because of their large size, eccentric kidney-shaped nucleus and prominent cytoplasmic granules, these cytotoxic cells, termed large granular lymphocytes (LGL), can be readily identified in Geimsa stained cytocentrafuge preparations. Unfortunately, the relatively low numbers of these cells in normal lymphoid tissues has made the detailed analysis of LGL quite difficult. Recently however, a number of investigators have reported both rodent and human leukemias or leukocytosis in which there was an abnormally high number of circulating lymphocytes with either the appearance and/or function of LGL. The present manuscript reviews this literature with an emphasis on the biological and clinical characteristics of this lymphoproliferative disease. Emphasis is also placed on the usefulness of these cells for the detailed analysis of LGL morphology and function.
    Critical Reviews in Oncology/Hematology 02/1985; 2(3):185-208. DOI:10.1016/S1040-8428(85)80002-0 · 4.03 Impact Factor
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    ABSTRACT: A 54-year-old asymptomatic male patient was followed for more than 7 y and presented a constant T cell lymphocytosis without skin involvement or bone marrow depression. No clinical or haematological aggravation was noted during this follow-up. Morphologically, the cells were large granular lymphocytes strongly positive for beta-D-glucuronidase, negative for acid phosphatase and with features of T cells on transmission and scanning electron microscopy. The immunological studies of the lymphocytes showed the following parameters: E rosettes+, mouse rosettes-, SmIg-, OKT3+, OKT4+, OKT8-, OKT6-, Ia-, TdT-, NK-, HTLV-, decreased PHA and PWM stimulation, no interleukin 2 production and failure to enhance Ig synthesis in a PWM driven system. The karyotype was normal. This case of chronic T cell lymphocytosis with large granular lymphocytes helper profile and defect of helper function, not reported in the literature, may correspond to a distinct entity in the heterogeneous group of chronic T cell disorders.
    Scandinavian journal of haematology 03/1985; 34(2):160-9. DOI:10.1111/j.1600-0609.1985.tb02250.x
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