Osseointegration and Its Experimental Background
Available from: María Carmen De Andrés
- "Typically, the approach is to harness the therapeutic potential of stem cells together with an appropriate biomaterial  . Ti has long been the gold standard for orthopaedic given the excellent biocompatibility, low corrosion, wear resistance and to promote osseointegration at the boneimplant interface . For the development of osseointegration the recruitment of cells with osteogenic potential is essential. "
[Show abstract] [Hide abstract]
To evaluate the osteo-regenerative potential of Titanium (Ti) modified by Light Amplification by Stimulated Emission of Radiation (LASER) beam (Yb-YAG) upon culture with human Skeletal Stem Cells (hSSCs(1)).
Human skeletal cell populations were isolated from the bone marrow of haematologically normal patients undergoing primary total hip replacement following appropriate consent. STRO-1(+) hSSC(1) function was examined for 10 days across four groups using discs: i) machined Ti surface group in basal media (Mb(2)), ii) machined Ti surface group in osteogenic media (Mo(3)), iii) LASER-modified Ti group in basal media (Lb(4)) and, iv) LASER-modified Ti group in osteogenic media (Lo(5)). Molecular analysis and qRT-PCR as well as functional analysis including biochemistry (DNA, Alkaline Phosphatase (ALP(6)) specific activity), live/dead immunostaining (Cell Tracker Green(CTG(7))/Ethidium Homodimer-1(EH-1(8))), and fluorescence staining (for vinculin and phalloidin) were undertaken. Inverted, confocal and Scanning Electron Microscopy (SEM) approaches were used to characterise cell adherence, proliferation, and phenotype.
Enhanced cell spreading and morphological rearrangement, including focal adhesions were observed following culture of hSSCs(1) on LASER surfaces in both basal and osteogenic conditions. Biochemical analysis demonstrated enhanced ALP(6) specific activity on the hSSCs(1)-seeded on LASER-modified surface in basal culture media. Molecular analysis demonstrated enhanced ALP(6) and osteopontin expression on titanium LASER treated surfaces in basal conditions. SEM, inverted microscopy and confocal laser scanning microscopy confirmed extensive proliferation and migration of human bone marrow stromal cells on all surfaces evaluated.
LASER-modified Ti surfaces modify the behaviour of hSSCs(1). In particular, SSC(1) adhesion, osteogenic gene expression, cell morphology and cytoskeleton structure were affected. The current studies show Ti LASER modification can enhance the osseointegration between Ti and skeletal cells, with important implications for orthopaedic application.
Biochemical and Biophysical Research Communications 10/2015; DOI:10.1016/j.bbrc.2015.10.013 · 2.30 Impact Factor
Available from: Purnima S Kumar
- "Root-form or endosseous implants are screwshaped devices made of titanium alloy that replace the root of a tooth. Stability is achieved through a process of osseointegration, which is defined as intimate structural and functional contact between the implant and surrounding bone (Branemark, 1983). Osseointegrated implants are connected to ceramic or metal crowns by structures called abutments, and are said to be " functionally loaded " when occlusal contact is established following crown placement. "
[Show abstract] [Hide abstract]
ABSTRACT: Peri-implant diseases (peri-implantitis and peri-implant mucositis) are bacterially driven infections. Peri-implantitis leads to aggressive bone resorption and eventual loss of the implant. Traditionally, peri-implantitis was regarded as microbially similar to periodontitis, and translocation of periodontal pathogens into the peri-implant crevice was considered as a critical factor in disease causation. However, evidence is emerging to suggest that the peri-implant and periodontal ecosystems differ in many important ways. The purpose of this review is to examine the evidence supporting microbial congruence and discordance in these two communities. Current evidence suggests that osseointegrated implants truly create unique microenvironments that force microbial adaptation and selection. Further studies that revisit the 'microbial reservoir' hypothesis and identify species that play an etiologic role in peri-implant disease and examine their transmission from teeth are needed. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
- "Biological processes associated with the osseointegration of endosseous dental implants have been extensively investigated in numerous preclinical and clinical studies   . The level of osseointegration is typically evaluated by histological assessment of bone to implant contact (BIC) areas in undecalcified hard tissue sections . One major drawback of nondecalcified sectioning, however, is a significant loss of biological * Corresponding author at: Department of Orthodontics, Westdeutsche Kieferklinik , Heinrich-Heine University, Moorenstr. "
[Show abstract] [Hide abstract]
ABSTRACT: We propose a novel 3D-2D registration approach for micro-computed tomography (μCT) and histology (HI), constructed for dental implant biopsies, that finds the position and normal vector of the oblique slice from μCT that corresponds to HI. During image pre-processing, the implants and the bone tissue are segmented using a combination of thresholding, morphological filters and component labeling. After this, chamfer matching is employed to register the implant edges and fine registration of the bone tissues is achieved using simulated annealing. The method was tested on n=10 biopsies, obtained at 20 weeks after non-submerged healing in the canine mandible. The specimens were scanned with μCT 100 and processed for hard tissue sectioning. After registration, we assessed the agreement of bone to implant contact (BIC) using automated and manual measurements. Statistical analysis was conducted to test the agreement of the BIC measurements in the registered samples. Registration was successful for all specimens and agreement of the respective binary images was high (median: 0.90, 1.-3. Qu.: 0.89-0.91). Direct comparison of BIC yielded that automated (median 0.82, 1.-3. Qu.: 0.75-0.85) and manual (median 0.61, 1.-3. Qu.: 0.52-0.67) measures from μCT were significant positively correlated with HI (median 0.65, 1.-3. Qu.: 0.59-0.72) between μCT and HI groups (manual: R(2)=0.87, automated: R(2)=0.75, p<0.001). The results show that this method yields promising results and that μCT may become a valid alternative to assess osseointegration in three dimensions.
Copyright © 2015 Elsevier Ltd. All rights reserved.
Computerized medical imaging and graphics: the official journal of the Computerized Medical Imaging Society 05/2015; 44. DOI:10.1016/j.compmedimag.2015.04.005 · 1.22 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.