Basement membrane glycoprotein laminin is an agglutinin.
ABSTRACT Laminin was purified to homogeneity from the extracellular matrix and soluble fraction of teratocarcinoma OTT6050 and also partially purified from the ascitic fluid of the mice carrying the teratocarcinoma. These laminin preparations were found to agglutinate trypsinized, glutaraldehyde-fixed rabbit erythrocytes. The hemagglutinating activity was inhibited by porcine gastric mucin, which invertase and mannan were not inhibitory. Heparin and heparan sulfate also inhibited the hemagglutination. Simple saccharides such as D-galactose, N-acetyl D-glucosamine, and N-acetyl D-galactosamine were not inhibitory, but D-glucosamine and D-galactosamine were. The hemagglutinating activity required Ca2+ and was dependent upon temperature. These results raised the possibility that laminin functions also in cell-cell interactions such as cell-cell adhesion. In addition, we report that laminin synthesized by the teratocarcinoma did not carry the large carbohydrate chain characteristic of early embryonic cells.
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ABSTRACT: Nagoya Journal of Medical Science, 57(3-4), 1994, p. 95-108
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ABSTRACT: The expression of two carbohydrate markers--namely, 4C9 antigen, which is an Lex antigen, and the Dolichos biflorus agglutinin (DBA) binding site, which is an N-acetylgalactosamine marker--was examined histochemically in tumors and adjacent nontumorous tissues of 102 cases of human lung carcinomas. In nontumorous tissues, the DBA binding site was expressed more frequently than 4C9 antigen, and the DBA binding site had a tendency to be expressed more significantly than in tumor cells. Adenocarcinomas and well-differentiated tumors had a tendency to more cell surface staining. Patients with tumors that expressed DBA binding sites but not 4C9 antigen (4C9-, DBA+) had fewer metastasis and significantly better prognoses than patients with tumors of other carbohydrate profiles. Better prognosis of patients with 4C9-, DBA+ tumors was observed in those with blood group A antigen and those without it, and the better prognosis also was observed in patients with Stage I and IIIA disease.Cancer 05/1992; 69(8):2084-90. DOI:10.1002/1097-0142(19920415)69:83.0.CO;2-S · 4.90 Impact Factor
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ABSTRACT: Embryoid bodies (EB) derived from teratocarcinoma OTT6050 are a useful model for the study of early embryogenesis and tumorigenesis and are found in two forms: cystic and simple. After injection of cystic EB intraperitoneally into isogenic 129/Sv mice, their growth and the expression of laminin (LAM) and fibronectin (FN) were studied at 5, 7 and 9 days post-injection. LAM was highly expressed on the internal face of endodermal and on some internal cells in cystic type EB and in both endodermal cell faces in simple EB. Only slight FN expression was observed in cystic and simple EB. Extracellular matrix proteins can be studied in this model.Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin 01/1991; 418(6):509-514. DOI:10.1007/BF01606501 · 2.56 Impact Factor