Anaerobic bacteria in dentoalveolar infections.
ABSTRACT The bacteriology of 57 dentoalveolar infections was studied using optimal techniques to collect, transport and process specimens. There was an average of 4 bacterial species per specimen, and only 1/3 of the specimens held aerobes. Among the aerobic bacteria, streptococci dominated and among the anaerobes the Gram-negative rods, Bacteroides ruminicola and Fusobacterium nucleatum, were most frequently isolated followed by Gram-positive cocci, in particular Streptococcus intermedius. All aerobic isolates were resistant to penicillins but sensitive to clindamycin and tinidazole. The other anaerobic isolates were sensitive to penicillins but showed varying susceptibility to erythromycin and doxycycline. Tinidazole was effective against all anaerobic Gram-negative rods. The presence of volatile fatty acids in pus from dentoalveolar infections was found to be of presumptive value for the diagnosis of anaerobic infections. Direct gas-liquid chromatographic analysis of pus is recommended as a routine procedure for preliminary diagnosis of anaerobic dentoalveolar infections.
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ABSTRACT: Objective: To determine the microbiology of odontogenic abscesses in patients with a history of empiric antibiotic administration.Patients and Methods: Percutaneous or permucosal aspiration of pus was carried out from closed nondraining abscesses using strict disinfection techniques and processing in an aerobic and anaerobic environment. Antibiotic sensitivity testing was performed by the Kirby-Bauer method.Results: A total of 120 isolates were reported from 100 study samples. In all, 27 different strains of microorganisms were isolated from the study group, with an average of 2.7 isolates per sample. Viridans streptococci were the most frequently isolated organisms. Of 7 antibiotics tested, penicillin G, clindamycin and ciprofoxacin had excellent activity against the isolates.Conclusions: Orofacial odontogenic abscesses in patients with a history of empiric antibiotic therapy predominantly contain Gram positive facultative organisms, especially viridans streptococci. The precise role of anaerobic organisms in persistent infections following empiric antibiotic therapy is debatable, and further investigations using molecular biological techniques are required. Penicillin G, as tested in this study, remains the drug of choice for treating odontogenic infections. The incidence of penicillin resistance was found to be negligible in this study.Asian Journal of Oral and Maxillofacial Surgery 12/2006; 18(4):272–279. DOI:10.1016/S0915-6992(06)80043-9
Article: Bacteriology of Orofacial Infection[Show abstract] [Hide abstract]
ABSTRACT: Objective: To establish the causative organisms of orofacial infections and their antimicrobial susceptibilities. Patients and Methods: In this retrospective chart review, 416 samples of pus were obtained from 409 patients with orofacial infection attending a dental school between 1994 and 1998 were sent for culture and susceptibility testing.Results: 109 samples grew normal oral flora and 228 samples grew pathogens. No organisms were isolated in 79 samples. The most common pathogens isolated were viridans streptococci (22.4%), Staphylococcus aureus (18.4%), enteric gram-negative bacteria (20.2%), and Pseudomonas aeruginosa (9.7%). All strains of viridans streptococci were susceptible to penicillin. Viridans streptococci and Staphylococcus aureus showed good susceptibilities to erythromycin of 85.7% and 87.1%, respectively. Of the enteric gram-negative bacteria, 47.8% were resistant to ampicillin. No anaerobic organisms were isolated in this study.Conclusions: The profile of aerobic isolates and their susceptibilities to antimicrobial agents should assist in selecting empirical therapy and directing therapy for orofacial infection. Inclusion of antimicrobial agents against anaerobic bacteria is recommended.Asian Journal of Oral and Maxillofacial Surgery 09/2005; 17(3):168–172. DOI:10.1016/S0915-6992(05)80045-7
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ABSTRACT: Purpose: To detect predominant bacteria associated with radicular cysts and discuss in light of the literature. Clinical materials were obtained from 35 radicular cysts by aspiration. Cultures were made from clinical materials by modern laboratory techniques, they underwent microbiologic analysis. The following are microorganisms isolated from cultures: Streptococcus milleri Group (SMG) (23.8%) [Streptococcus constellatus (19.1%) and Streptococcus anginosus (4.7%)], Streptococcus sanguis (14.3%), Streptococcus mitis (4.7%), Streptococcus cremoris (4.7%), Peptostreptococcus pevotii (4.7%), Prevotella buccae (4.7%), Prevotella intermedia (4.7%), Actinomyces meyeri (4.7%), Actinomyces viscosus (4.7%), Propionibacterium propionicum (4.7%), Bacteroides capillosus (4.7%), Staphylococcus hominis (4.7%), Rothia denticariosa (4.7%), Gemella haemolysans (4.7%), and Fusobacterium nucleatum (4.7%). Results of this study demonstrated that radicular cysts show a great variety of anaerobic and facultative anaerobic bacterial flora. It was observed that all isolated microorganisms were the types commonly found in oral flora. Although no specific microorganism was found, Streptococcus spp. bacteria (47.5%) -- especially SMG (23.8%) -- were predominantly found in the microorganisms isolated. Furthermore, radicular cysts might be polymicrobial originated. Although radicular cyst is an inflammatory cyst, some radicular cyst fluids might be sterile.Head & Face Medicine 09/2013; 9(1):25. DOI:10.1186/1746-160X-9-25 · 0.87 Impact Factor