The Influence Of Ionic Strength, Ph And A Protein Layer On The Interaction Between Streptococcus Mutans And Glass Surfaces

Journal of general microbiology 03/1983; 129(2):439-45. DOI: 10.1099/00221287-129-2-439
Source: PubMed


The initial interaction between Streptococcus mutans and hard surfaces has been investigated using a rotating disc technique. The deposition to clean and BSA-coated glass of two strains of S. mutans, FA-1 (serotype b) and KPSK2 (serotype c), which exhibit different surface properties, was studied. Organisms were harvested from cultures grown in a chemostat at a dilution rate of 0.06 h-1 and suspended in NaCl solutions of defined ionic strengths and pH values. The deposition of both strains showed a strong dependence on electrolyte concentration, particularly at low ionic strengths, which was inversely related to the zeta potentials of the organisms. Similarly, the ionic strength at which maximum deposition was first noted (critical coagulation concentration) for the two strains correlated with their relative potentials. Deposition was insensitive to changes in pH at an electrolyte concentration of 0.05 M. The maximum observed deposition did not approach values predicted by theory, suggesting that a further barrier to deposition, other than electrostatic repulsion, might exist. Under all experimental conditions, some of the deposited bacteria were observed to be oscillating, suggesting that they were held at a distance from the collector surface. The cells did not, however, appear to be deposited in a secondary minimum predicted by DLVO theory hence it may be that long-range polymer interactions are also involved in the deposition of these organisms.

13 Reads
  • Source
    • "Previous studies investigating bacterial attachment and inhibition by phytochemicals to components of the oral cavity have focused on attachment to hard surfaces such as enamel [10,13,14]. Attachment of bacteria to soft tissues in the mouth can also initiate disease and for this reason we investigated the effects of tea extracts and tea components on attachment of oral pathogenic bacteria to an immortalized line of connective gingival fibroblasts in vitro. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Tea has been suggested to promote oral health by inhibiting bacterial attachment to the oral cavity. Most studies have focused on prevention of bacterial attachment to hard surfaces such as enamel. Findings This study investigated the effect of five commercial tea (green, oolong, black, pu-erh and chrysanthemum) extracts and tea components (epigallocatechin gallate and gallic acid) on the attachment of five oral pathogens (Streptococcus mutans ATCC 25175, Streptococcus mutans ATCC 35668, Streptococcus mitis ATCC 49456, Streptococcus salivarius ATCC 13419 and Actinomyces naeslundii ATCC 51655) to the HGF-1 gingival cell line. Extracts of two of the teas (pu-erh and chrysanthemum) significantly (p < 0.05) reduced attachment of all the Streptococcus strains by up to 4 log CFU/well but effects of other teas and components were small. Conclusions Pu-erh and chrysanthemum tea may have the potential to reduce attachment of oral pathogens to gingival tissue and improve the health of oral soft tissues.
    BMC Research Notes 04/2013; 6(1):143. DOI:10.1186/1756-0500-6-143
  • Source
    • "Several studies have indicated that bacterial adhesion negatively correlated with bacterial zeta potentials (van Loosdrecht et al 1989; Tsuneda et al 2003; Li and Logan 2004; Tsuneda et al 2004; Eboigbodin et al 2005), conversely, other studies have been reported that there are no relationship between the two parameters (Abbot et al 1983; Hogt et al 1985; Harkes et al 1991). A review by Donlan (2002) has discussed the contribution of bacterial fi mbriae in the surface attachment mechanism. "
    [Show abstract] [Hide abstract]
    ABSTRACT: This study reports the adhesion behavior of two bacterial species, Vibrio fischeri and Escherichia coli, to the photoresistant poly(tert-butyl methacrylate) (P(tBMA)) polymer surface. The data has demonstrated that ultraviolet irradiation of P(tBMA) was able to provide control over bacterial adhesion tendencies. Following photolithography, several of the surface characteristics of P(tBMA) were found to be altered. Atomic force microscopy analysis indicated that photolithographically modified P(tBMA) (henceforth termed 'modified polymer') appeared as a 'nanosmooth' surface with an average surface roughness of 1.6 nm. Although confocal laser scanning microscopy and scanning electron microscopy analysis clearly demonstrated that V. fischeri and E. coli presented largely different patterns of attachment in order to adhere to the same surfaces, both species exhibited a greater adhesion propensity towards the 'nanosmooth' surface. The adhesion of both species to the modified polymer surface appeared to be facilitated by an elevated production of extracellular polymeric substances when in contact with the substrate.
    Nanotechnology, Science and Applications 01/2008; 1:33-44.
  • Source
    • "lates between 282 and 323 mOsm kg −1 which can be converted to 0·97% NaCl equivalents in open-eye tears, and to 0·89% in closed-eye tears (Terry and Hill 1978). An increase in salt concentration leads to a compression of the electric double layers around surfaces in suspension, which in turn affect the equilibrium between the attractive van der Waal's forces and repulsive electrostatic forces (Abbott et al. 1983). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. The salt concentration of media is an important parameter controlling microbial adhesion. Salinity varies from 0.97% NaCl equivalents in the open eye to 0.89% in the closed eye state. In this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) NaCl on adhesion of ocular bacteria to soft contact lenses was investigated using a static adhesion assay. Pseudomonas aeruginosa was found to adhere to lenses in significantly greater amounts than Serratia marcescens, Flavobacterium meningosepticum, Stenotrophomonas maltophilia and Staphylococcus intermedius. Increasing NaCl from 0.8% to 1.0% (w/v) increased adhesion of all bacteria tested. This adhesion was strong since the organisms could not be removed by washing in low ionic buffer. Adhesion of these organisms did not correlate with their cell surface properties as determined by bacterial adhesion to hydrocarbons (BATH) and retention on sepharose columns.
    Journal of Applied Microbiology 07/1998; 84(6):950-8. DOI:10.1046/j.1365-2672.1998.00427.x · 2.48 Impact Factor
Show more


13 Reads
Available from