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The Influence Of Ionic Strength, Ph And A Protein Layer On The Interaction Between Streptococcus Mutans And Glass Surfaces

Journal of general microbiology 03/1983; 129(2):439-45. DOI: 10.1099/00221287-129-2-439
Source: PubMed

ABSTRACT The initial interaction between Streptococcus mutans and hard surfaces has been investigated using a rotating disc technique. The deposition to clean and BSA-coated glass of two strains of S. mutans, FA-1 (serotype b) and KPSK2 (serotype c), which exhibit different surface properties, was studied. Organisms were harvested from cultures grown in a chemostat at a dilution rate of 0.06 h-1 and suspended in NaCl solutions of defined ionic strengths and pH values. The deposition of both strains showed a strong dependence on electrolyte concentration, particularly at low ionic strengths, which was inversely related to the zeta potentials of the organisms. Similarly, the ionic strength at which maximum deposition was first noted (critical coagulation concentration) for the two strains correlated with their relative potentials. Deposition was insensitive to changes in pH at an electrolyte concentration of 0.05 M. The maximum observed deposition did not approach values predicted by theory, suggesting that a further barrier to deposition, other than electrostatic repulsion, might exist. Under all experimental conditions, some of the deposited bacteria were observed to be oscillating, suggesting that they were held at a distance from the collector surface. The cells did not, however, appear to be deposited in a secondary minimum predicted by DLVO theory hence it may be that long-range polymer interactions are also involved in the deposition of these organisms.

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    • "Several studies have indicated that bacterial adhesion negatively correlated with bacterial zeta potentials (van Loosdrecht et al 1989; Tsuneda et al 2003; Li and Logan 2004; Tsuneda et al 2004; Eboigbodin et al 2005), conversely, other studies have been reported that there are no relationship between the two parameters (Abbot et al 1983; Hogt et al 1985; Harkes et al 1991). A review by Donlan (2002) has discussed the contribution of bacterial fi mbriae in the surface attachment mechanism. "
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    • "lates between 282 and 323 mOsm kg −1 which can be converted to 0·97% NaCl equivalents in open-eye tears, and to 0·89% in closed-eye tears (Terry and Hill 1978). An increase in salt concentration leads to a compression of the electric double layers around surfaces in suspension, which in turn affect the equilibrium between the attractive van der Waal's forces and repulsive electrostatic forces (Abbott et al. 1983). "
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