The Influence Of Ionic Strength, Ph And A Protein Layer On The Interaction Between Streptococcus Mutans And Glass Surfaces

Journal of general microbiology 03/1983; 129(2):439-45. DOI: 10.1099/00221287-129-2-439
Source: PubMed

ABSTRACT The initial interaction between Streptococcus mutans and hard surfaces has been investigated using a rotating disc technique. The deposition to clean and BSA-coated glass of two strains of S. mutans, FA-1 (serotype b) and KPSK2 (serotype c), which exhibit different surface properties, was studied. Organisms were harvested from cultures grown in a chemostat at a dilution rate of 0.06 h-1 and suspended in NaCl solutions of defined ionic strengths and pH values. The deposition of both strains showed a strong dependence on electrolyte concentration, particularly at low ionic strengths, which was inversely related to the zeta potentials of the organisms. Similarly, the ionic strength at which maximum deposition was first noted (critical coagulation concentration) for the two strains correlated with their relative potentials. Deposition was insensitive to changes in pH at an electrolyte concentration of 0.05 M. The maximum observed deposition did not approach values predicted by theory, suggesting that a further barrier to deposition, other than electrostatic repulsion, might exist. Under all experimental conditions, some of the deposited bacteria were observed to be oscillating, suggesting that they were held at a distance from the collector surface. The cells did not, however, appear to be deposited in a secondary minimum predicted by DLVO theory hence it may be that long-range polymer interactions are also involved in the deposition of these organisms.

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    • "Several studies have indicated that bacterial adhesion negatively correlated with bacterial zeta potentials (van Loosdrecht et al 1989; Tsuneda et al 2003; Li and Logan 2004; Tsuneda et al 2004; Eboigbodin et al 2005), conversely, other studies have been reported that there are no relationship between the two parameters (Abbot et al 1983; Hogt et al 1985; Harkes et al 1991). A review by Donlan (2002) has discussed the contribution of bacterial fi mbriae in the surface attachment mechanism. "
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    ABSTRACT: This study reports the adhesion behavior of two bacterial species, Vibrio fischeri and Escherichia coli, to the photoresistant poly(tert-butyl methacrylate) (P(tBMA)) polymer surface. The data has demonstrated that ultraviolet irradiation of P(tBMA) was able to provide control over bacterial adhesion tendencies. Following photolithography, several of the surface characteristics of P(tBMA) were found to be altered. Atomic force microscopy analysis indicated that photolithographically modified P(tBMA) (henceforth termed 'modified polymer') appeared as a 'nanosmooth' surface with an average surface roughness of 1.6 nm. Although confocal laser scanning microscopy and scanning electron microscopy analysis clearly demonstrated that V. fischeri and E. coli presented largely different patterns of attachment in order to adhere to the same surfaces, both species exhibited a greater adhesion propensity towards the 'nanosmooth' surface. The adhesion of both species to the modified polymer surface appeared to be facilitated by an elevated production of extracellular polymeric substances when in contact with the substrate.
    Nanotechnology, Science and Applications 01/2008; 1:33-44.
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    • "lates between 282 and 323 mOsm kg −1 which can be converted to 0·97% NaCl equivalents in open-eye tears, and to 0·89% in closed-eye tears (Terry and Hill 1978). An increase in salt concentration leads to a compression of the electric double layers around surfaces in suspension, which in turn affect the equilibrium between the attractive van der Waal's forces and repulsive electrostatic forces (Abbott et al. 1983). "
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    ABSTRACT: Adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. The salt concentration of media is an important parameter controlling microbial adhesion. Salinity varies from 0.97% NaCl equivalents in the open eye to 0.89% in the closed eye state. In this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) NaCl on adhesion of ocular bacteria to soft contact lenses was investigated using a static adhesion assay. Pseudomonas aeruginosa was found to adhere to lenses in significantly greater amounts than Serratia marcescens, Flavobacterium meningosepticum, Stenotrophomonas maltophilia and Staphylococcus intermedius. Increasing NaCl from 0.8% to 1.0% (w/v) increased adhesion of all bacteria tested. This adhesion was strong since the organisms could not be removed by washing in low ionic buffer. Adhesion of these organisms did not correlate with their cell surface properties as determined by bacterial adhesion to hydrocarbons (BATH) and retention on sepharose columns.
    Journal of Applied Microbiology 07/1998; 84(6):950-8. DOI:10.1046/j.1365-2672.1998.00427.x · 2.39 Impact Factor
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