Physicochemical and serological characterization of two rhabdoviruses isolated from eels.
ABSTRACT Summary Two bullet-shaped viruses, isolated from eels, have been characterized physico-chemically, and their serological relationship to the other fish rhabdoviruses has been determined. Preparations of each virus contain truncated as well as bullet-shaped particles and the RNAs from these sediment at 18S and 4IS, respectively. As determined by PAGE, the virion polypeptide patterns for both viruses were indistinguishable, but were found to differ from those of the other fish rhabdoviruses. Using vesicular stomatitis virus polypeptides as internal markers, the molecular weights of the eel virus polypeptides were calculated to be > 150,000, 64,000, 49,000, 47,000 and 26,000. The two viruses are closely related to each other antigenically, but show no relationship to the other fish rhabdoviruses in neutralization tests.Copyright © 1980 S. Karger AG, Basel
- SourceAvailable from: Niels Jørgen OlesenDiseases of Aquatic Organisms 01/1993; 16:171-179. · 1.59 Impact Factor
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ABSTRACT: Wild freshwater eel populations have dramatically declined in recent last decades in Europe and America, partially through the impact of several factors including the widespread of infectious diseases. The Anguillid rhabdoviruses Eel Virus European X (EVEX) and Eel Virus American (EVA) play potentially a role in this decline, even if their real contribution is still unclear. In this study, we investigate the evolutionary dynamics and genetic diversity of Anguillid rhabdoviruses by analyzing sequences from the glycoprotein (G), nucleoprotein (N), and phosphoprotein (P) genes of 57 viral strains collected from 7 countries over 40 years using Maximum likelihood and Bayesian approaches. Phylogenetic trees from the three genes are congruent and allow to clearly distinguish two monophyletic groups, European and American. Results of nucleotide substitution rates per site per year indicate that the P gene is subjected to evolve more rapidly. The nucleotide diversity observed is low (2-3 %) for the three genes, with a significantly higher variability within the P gene which encodes multiple proteins from a single genomic RNA sequence, particularly a small C protein. This putative C protein could be a potential molecular marker suitable for characterization of distinct genotypes within Anguillid rhabdoviruses. This study provides to our knowledge the first molecular characterization of EVA, brings new insights on the evolutionary dynamics of two genotypes of Anguillid rhabdovirus, and is a baseline for further investigations on the tracking of its spread.Journal of General Virology 07/2014; · 3.53 Impact Factor
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ABSTRACT: A novel large strain sensor has been developed using a silicone/nickel nanostrand/nickel coated carbon fiber nanocomposite system. The effect of conductive filler volume fraction on the piezoresistive response of the nanocomposite sensor has been studied in order to determine the optimal composition for use in large strain/motion sensing applications. Electromechanical testing of various compositions revealed that optimum performance was achieved using 11vol% nickel nanostrands with 2vol% nickel coated carbon fiber in the silicone matrix. Initial results indicate that this nanocomposite is capable of sensing strains of over 40% elongation.Sensors and Actuators A Physical 03/2011; 166(1):40-47. · 1.94 Impact Factor