Cell proliferation in dysplasia of the prostate: analysis by PCNA immunostaining.

Department of Urology, Stanford University School of Medicine, California, USA.
The Prostate (Impact Factor: 3.57). 12/1995; 27(5):258-68.
Source: PubMed


Patterns of cell proliferation in the prostate were compared between benign epithelium and dysplasia. Proliferating cell nuclear antigen (PCNA) immunostaining was used to quantitate proliferation, and basal cells were tallied separately from secretory cells with the aid of keratin immunostaining. Using a novel technique, absolute cell densities (cells/mm) were determined and used to calculate growth fractions. In benign epithelium, 83% of PCNA+ cells were basal cells, while only 7% of PCNA+ cells in dysplasia were basal cells and there was a clear separation between groups. This dramatic shift of the proliferative compartment to the secretory cells in dysplasia was accompanied only by a moderate increase in overall secretory cell density and moderate reduction in basal cell density, but these ranges overlapped those of benign epithelium. The median PCNA+ secretory cell "growth fraction" was 0.12% in benign epithelium and 1.06% in dysplasia. The findings presented give further support to the concept that dysplasia represents an evolutionary stage in the malignant transformation of prostatic epithelium. The patterns of change in PCNA immunostaining may reflect certain aspects of the biologic nature of malignant transformation.

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    • "In contrast, a luminal progenitor cell population expressing telomerase could expand and give rise to terminally differentiated luminal cells, which do not replicate, maintaining a telomere length similar to that of the luminal progenitors. Our data is in agreement with both Bonkhoff et al [10] and McNeal et al [11] with respect to the existence of a proliferative basal compartment in which the potential luminal progenitors derived from basal SCs exhibit characteristics of both basal and luminal phenotypes, analogous to castration-resistant Nkx3.1-expressing cells in mice [12]. The second implication of our model is that local inhibition of telomerase in BPH could be an alternative therapeutic strategy, as it will inhibit the proliferation both luminal and basal epithelial progenitors but perhaps not that of stromal cells. "
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    • "Most investigators report that almost every cell in primary cultures derived from normal tissues expresses these basal cytokeratins (Brawer et al. 1986, Gao et al. 2001). Proliferation is also confined to the basal cells of normal tissues (McNeal et al. 1995, Kyprianou et al. 1996) and, similarly, primary cultures of normal prostatic epithelial cells are proliferative. "
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