Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver.
ABSTRACT The effect of carrot extract on carbon tetrachloride (CCl4)-induced acute liver damage was evaluated. The increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by CCl4-induction were significantly lowered due to pretreatment with the extract. The extract also decreased the elevated serum bilirubin and urea content due to CCl4 administration. Increased activities of hepatic 5'-nucleotidase, acid phosphatase, acid ribonuclease and decreased levels of succinic dehydrogenase, glucose-6-phosphatase and cytochrome P-450 produced by CCl4 were reversed by the extract in a dose-responsive way. Results of this study revealed that carrot could afford a significant protective action in the alleviation of CCl4-induced hepatocellular injury.
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ABSTRACT: The present study was to estimate preliminary photochemical evaluation and in vitro antioxidant of aerial parts extracts of Daucus aureus by using both solvents like ethanol and water. Preliminary phytochemical analysis reveals the presence of tannins, flavanoids, steroids and terpenoids. The extracts were screened for its potential antioxidant activity using DPPH free radical scavenging activity. The reducing power extract was also determined ascorbic acid was used as a standard and positive control for aerial parts analysis. Ethanol extract showed significant activity with DPPH (2, 2-Diphenyl-1-picryl-hydrazyl) radical scavenging compared to standard antioxidant. However, increasing the concentration of the extracts resulted in increased ferric reducing antioxidant power for both extracts tested. From the analyses, ethanolic extract had the highest total phenolic content. Finally, a relationship was observed between the antioxidant activity potential and total phenolic and flavonoid levels of the extract. The results were concluded that extracts have a potential source of antioxidants of natural origin.International Journal of Traditional and Herbal Medicine. 09/2013; 1(1):2.
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ABSTRACT: Abstract Context: Daucus carota Linn (Apiaceae), a useful vegetable, is traditionally used in treating kidney and hepatic dysfunctions. Objective: To evaluate the protective and curative potential of D. carota root extract on renal ischemia reperfusion injury in rats. Materials and methods: Wistar rats were selected with 8 + 8 groups (n = 6). Renal pedicles of rats were occluded for 45 min and allowed for reperfusion period. In protective and curative studies, 14 days prior and 14 days after the induction of ischemia/reperfusion (I/R), rats received petroleum ether extract (PEE 250 and 500 mg/kg), fractional methanol extract (FME 250 and 500 mg/kg) and direct methanol extract (DME 250 and 500 mg/kg) of Daucus carota root, orally, once daily. Results: PEE at a dose of 500 mg/kg significantly (p < 0.001) reduced the levels of serum creatinine (0.853-3.090 mg/dl), uric acid (1.300-3.500 mg/dl) and urea (58.26-132.00 mg/dl) compared to disease control. FME at a dose of 500 mg/kg body weight significantly (p < 0.001) reduced the levels of serum creatinine (0.960-3.090 mg/dl), uric acid (1.700-3.500 mg/dl) and urea (77.17-132.00 mg/dl) compared to disease control. DME at a dose of 500 mg/kg body weight significantly (p < 0.001) reduced the levels of serum creatinine (1.173-3.090 mg/dl), uric acid (2.267-3.500 mg/dl) and urea (84.75-132.00 mg/dl) compared to disease control. Discussion and conclusion: Findings demonstrate that postconditioning with the D. carota root extract significantly improves kidney function in I/R rats.Pharmaceutical Biology 04/2013; · 1.21 Impact Factor
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ABSTRACT: Through a hepatoprotective bioassay-guided fractionation of the methanol extract from Centaurea pallescens Del. (Asteraceae), a new acylated flavonoid triglycoside, 4′-methoxy kaempferol 3-O-[α-l-rhamnopyranosyl-(1→3)-(2-O-E-p-coumaroyl)] β-d-glucopyranoside-7-O-(4-O-E-p-coumaroyl) α-l-rhamnopyranoside (1) was isolated from the highly active aqueous fraction. In addition, six known compounds were isolated from both aqueous (2–3) and ethyl acetate soluble fractions (4–7). The structure of (1) was determined by comprehensive analysis of 1D and 2D NMR and mass spectral data. The protective effects of the methanol extract of C. pallescens and its fractions on the human hepatoma cell line were evaluated using Silymarin as a positive control. Hepatoprotection was assessed through determination of aspartate aminotransferase (AST), alanine transaminase (ALT), and superoxide dismutase (SOD) activities in addition to glutathione (GSH) levels before and after incubating the cells with carbon tetrachloride. Compound 1, the major constituent of the aqueous fraction, showed a significant cytoprotection at 100 μg/mL as evidenced by decreasing ALT activity to 18.6 ± 0.12, and enhancing SOD activity to 264.6 ± 4.3 U/mL. Meanwhile, compound 2 at 10 μg/mL decreased AST activity to 5.8 ± 2.4 U/mL. Moreover, Compounds 2 and 3 at 1,000 μg/mL significantly enhanced GSH levels. In conclusion, the protective effects of C. pallescens extract, its fractions and compounds 1–3 are concluded to be partly mediated by its antioxidant activity.Medicinal Chemistry Research 12/2013; · 1.61 Impact Factor