Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. J Ethnopharmacol

Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Journal of Ethnopharmacology (Impact Factor: 3). 08/1995; 47(2):69-74. DOI: 10.1016/0378-8741(95)01254-B
Source: PubMed

ABSTRACT The effect of carrot extract on carbon tetrachloride (CCl4)-induced acute liver damage was evaluated. The increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by CCl4-induction were significantly lowered due to pretreatment with the extract. The extract also decreased the elevated serum bilirubin and urea content due to CCl4 administration. Increased activities of hepatic 5'-nucleotidase, acid phosphatase, acid ribonuclease and decreased levels of succinic dehydrogenase, glucose-6-phosphatase and cytochrome P-450 produced by CCl4 were reversed by the extract in a dose-responsive way. Results of this study revealed that carrot could afford a significant protective action in the alleviation of CCl4-induced hepatocellular injury.

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    • "Moreover, rosemary provide protection against hepatotoxins by enhancing the functioning of the hepatic antioxidant defense system (Ip and Ko, 1996), inhibiting biosynthesis of cytochrome p 450 (Rao and Misra, 1998) and preventing LPO (Malo et al., 2011). Additionally, rosemary can help in stabilizing hepatocellular membrane, enhancing protein biosynthesis (Lin et al., 1999), decreasing the leakage of marker enzymes into the circulation, interfering with the microsomal activation of PAHs and/or accelerating detoxification (Bishayee et al., 1995). In agreement with the present study, Guti errez et al. (2009) and Sotelo-Felix et al. (2002) suggested that R. officinalis therapy, acting as an antioxidant and/or a free radical scavenger, can preserve cellular integrity and counteract the severe damage induced by carbon tetrachloride (CCl 4 ). "
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    ABSTRACT: Coal tar is a significant product generated from coal pyrolysis. Coal tar can be utilized as raw materials for various industries. It is also a type of raw material from which phenols, naphthalenes, and anthracene can be extracted. The present study was designed to investigate the possibility of coal tar creosote to induce oxidative stress and biochemical perturbations in rat liver and the role of rosemary (Rosmarinus officinalis) in ameliorating its toxic effects. Male Wister Albino rats were randomly divided into four groups of seven each, group I served as control; group II treated with rosemary (10 mL of water extract/kg BW for 21 days), group III received coal tar creosote (200 mg/4 mL olive oil/kg BW for 3 days), and group IV treated with both rosemary and coal tar creosote. The administration of coal tar creosote significantly caused elevation in lipid peroxidation (LPO) and reduction in the activities of glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST). A significant decrease in reduced glutathione (GSH) content was also observed. Liver aminotransferases aspartate transaminase (AST) and alanine transaminase (ALT)] and alkaline phosphatase (AlP) were significantly decreased while lactate dehydrogenase (LDH) was increased. Rosemary pretreatment to coal tar creosote-treated rats decreased LPO level and normalized GPx, GR, SOD, CAT, and GST activities, while GSH content was increased. Also, liver AST, ALT, AlP, and LDH were maintained near normal level due to rosemary treatment. In conclusion, rosemary has beneficial effects and could be able to antagonize coal tar creosote toxicity. © 2014 Wiley Periodicals, Inc. Environ Toxicol, 2014.
    Environmental Toxicology 07/2014; DOI:10.1002/tox.22024 · 3.20 Impact Factor
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    • "It is widely accepted that, in hepatic parenchyma cells, cytochrome P450-dependant monooxygenases convert the accumulated CCl4 into CCl3 radicals. In addition to the alkylation of cellular proteins, CCl3 attacks the polyunsaturated fatty acids to produce lipid peroxides that are responsible for the hepatotoxicity and alteration of hepatic enzyme levels [37]. The disturbance of hepatocytic transport function during hepatic injury causes an altered permeability of the membrane leading to the leakage of enzymes from the cells [38], thus resulting in the reduction of the ALT, AST, and ALP levels in the hepatic cells and elevation of their levels in the serum [20]. "
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    ABSTRACT: Oxidative stress, lipid peroxidation, and transaminase reactions are some of the mechanisms that can lead to liver dysfunction. A time-dependent study was designed to evaluate the ability of silymarin (SLN) and glycyrrhizin (GLN) in different dosage regimens to lessen oxidative stress in the rats with hepatic injury caused by the hepatotoxin carbon tetrachloride. Wistar male albino rats (n = 60) were randomly assigned to six groups. Group A served as a positive control while groups B, C, D, E, and F received a dose of CCl4 (50% solution of CCl4 in liquid paraffin, 2 mL/kg, intraperitoneally) twice a week to induce hepatic injury. Additionally, the animals received SLN and GLN in different doses for a period of six weeks. CCl4 was found to induce hepatic injury by significantly increasing serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and thiobarbituric acid reactive substances while decreasing total protein and the activities of reduced glutathione, superoxide dismutase, and catalase. Treatment with various doses of SLN and GLN significantly reduced ALT, AST, ALP, and TBARS levels and increased GSH, SOD, and CAT levels. Our findings indicated that SLN and GLN have hepatoprotective effects against oxidative stress of the liver.
    Evidence-based Complementary and Alternative Medicine 03/2014; 2014:641597. DOI:10.1155/2014/641597 · 1.88 Impact Factor
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    • "The antioxidative properties of some vegetables and fruits are partly due to the low molecular weight phenolic compounds, which are known to be potent as antioxidants (Wang et al., 1999). Daucus is used as antibacterial, stimulant (Emilio, 1994), antiseptic, carminative, diuretic, hepatoprotective (Bishayee et al., 1995), antisteroidogenic (Majumder et al., 1997) and anti-inflammatory (Porchezhian et al., 2000). The substances in carrots have a diuretic effect; help to ease an irritated gastrointestinal system, and help resistance to cancer (Radulovicét al., 2011).A great number of species and sub-species of Daucus genus have been tested for their antioxidant and antimicrobial activities during the last decade; however, there are still many others species of Daucus, which were not yet examined. "
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    ABSTRACT: The present study was to estimate preliminary photochemical evaluation and in vitro antioxidant of aerial parts extracts of Daucus aureus by using both solvents like ethanol and water. Preliminary phytochemical analysis reveals the presence of tannins, flavanoids, steroids and terpenoids. The extracts were screened for its potential antioxidant activity using DPPH free radical scavenging activity. The reducing power extract was also determined ascorbic acid was used as a standard and positive control for aerial parts analysis. Ethanol extract showed significant activity with DPPH (2, 2-Diphenyl-1-picryl-hydrazyl) radical scavenging compared to standard antioxidant. However, increasing the concentration of the extracts resulted in increased ferric reducing antioxidant power for both extracts tested. From the analyses, ethanolic extract had the highest total phenolic content. Finally, a relationship was observed between the antioxidant activity potential and total phenolic and flavonoid levels of the extract. The results were concluded that extracts have a potential source of antioxidants of natural origin.
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