Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver.
ABSTRACT The effect of carrot extract on carbon tetrachloride (CCl4)-induced acute liver damage was evaluated. The increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by CCl4-induction were significantly lowered due to pretreatment with the extract. The extract also decreased the elevated serum bilirubin and urea content due to CCl4 administration. Increased activities of hepatic 5'-nucleotidase, acid phosphatase, acid ribonuclease and decreased levels of succinic dehydrogenase, glucose-6-phosphatase and cytochrome P-450 produced by CCl4 were reversed by the extract in a dose-responsive way. Results of this study revealed that carrot could afford a significant protective action in the alleviation of CCl4-induced hepatocellular injury.
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ABSTRACT: Through a hepatoprotective bioassay-guided fractionation of the methanol extract from Centaurea pallescens Del. (Asteraceae), a new acylated flavonoid triglycoside, 4′-methoxy kaempferol 3-O-[α-l-rhamnopyranosyl-(1→3)-(2-O-E-p-coumaroyl)] β-d-glucopyranoside-7-O-(4-O-E-p-coumaroyl) α-l-rhamnopyranoside (1) was isolated from the highly active aqueous fraction. In addition, six known compounds were isolated from both aqueous (2–3) and ethyl acetate soluble fractions (4–7). The structure of (1) was determined by comprehensive analysis of 1D and 2D NMR and mass spectral data. The protective effects of the methanol extract of C. pallescens and its fractions on the human hepatoma cell line were evaluated using Silymarin as a positive control. Hepatoprotection was assessed through determination of aspartate aminotransferase (AST), alanine transaminase (ALT), and superoxide dismutase (SOD) activities in addition to glutathione (GSH) levels before and after incubating the cells with carbon tetrachloride. Compound 1, the major constituent of the aqueous fraction, showed a significant cytoprotection at 100 μg/mL as evidenced by decreasing ALT activity to 18.6 ± 0.12, and enhancing SOD activity to 264.6 ± 4.3 U/mL. Meanwhile, compound 2 at 10 μg/mL decreased AST activity to 5.8 ± 2.4 U/mL. Moreover, Compounds 2 and 3 at 1,000 μg/mL significantly enhanced GSH levels. In conclusion, the protective effects of C. pallescens extract, its fractions and compounds 1–3 are concluded to be partly mediated by its antioxidant activity.Medicinal Chemistry Research 12/2013; · 1.61 Impact Factor
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ABSTRACT: Oxidative stress, lipid peroxidation, and transaminase reactions are some of the mechanisms that can lead to liver dysfunction. A time-dependent study was designed to evaluate the ability of silymarin (SLN) and glycyrrhizin (GLN) in different dosage regimens to lessen oxidative stress in the rats with hepatic injury caused by the hepatotoxin carbon tetrachloride. Wistar male albino rats (n = 60) were randomly assigned to six groups. Group A served as a positive control while groups B, C, D, E, and F received a dose of CCl4 (50% solution of CCl4 in liquid paraffin, 2 mL/kg, intraperitoneally) twice a week to induce hepatic injury. Additionally, the animals received SLN and GLN in different doses for a period of six weeks. CCl4 was found to induce hepatic injury by significantly increasing serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and thiobarbituric acid reactive substances while decreasing total protein and the activities of reduced glutathione, superoxide dismutase, and catalase. Treatment with various doses of SLN and GLN significantly reduced ALT, AST, ALP, and TBARS levels and increased GSH, SOD, and CAT levels. Our findings indicated that SLN and GLN have hepatoprotective effects against oxidative stress of the liver.Evidence-based Complementary and Alternative Medicine 01/2014; 2014:641597. · 1.72 Impact Factor
- Antioxidants. 08/2014;