Chapter 7 Flow Cytometry Crossmatching for Solid Organ Transplantation

Department of Pathology, Emory University Hospital, Atlanta, Georgia 30322.
Methods in cell biology (Impact Factor: 1.42). 02/1994; 41:103-19. DOI: 10.1016/S0091-679X(08)61712-4
Source: PubMed
22 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: A flow cytometric method of simultaneously screening both HLA Class I and Class II panel reactive antibodies (PRA) was developed using a pool of 30 different Class I and 30 different Class II microbeads coated with purified HLA antigens. The antibodies in the serum that react specifically to the coated HLA antigens are detected by using a FITC-conjugated antibody against human IgG. Percent PRA can be determined by the percentage of microbeads that react positively to the serum. There is no cross-reactivity between the Class I and Class II microbeads. A mixture of Class I and Class II microbeads can be distinguished by their different fluorescent properties on the flow cytometry analysis. Thus, both Class I and Class II PRA can be detected from a single tube reaction.
    Human Immunology 06/1998; 59(5):313-22. DOI:10.1016/S0198-8859(98)00020-2 · 2.14 Impact Factor

  • Transplantation Proceedings 02/1999; 31(1-2):282-4. DOI:10.1016/S0041-1345(98)01628-5 · 0.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study was designed to investigate the clinical relevance of donor-specific antibodies (DS-Abs) and their influence on graft survival. Among 106 patients who underwent cadaveric kidney donor transplantation and were monitored by flow cytometry crossmatch (FCXM) during the 1st posttransplantation year, 25 (23.6%) resulted positive for DS-Ab production. During a 2-year follow up only 12 of the 81 FCXM-negative patients (14.8%) suffered rejection vs 17 of 25 FCXM-positive patients (68%; P = 0.00001). Correlating graft loss to DS-Ab production, 9 FCXM-positive patients lost the graft vs only 1 among the FCXM-negative patients. A worse graft function was evidenced in FCXM-positive subjects who had also suffered rejection episodes than in those which had acute rejection but did not produce DS-Abs. A high incidence of HLA-AB mismatches was found in FCXM-positive subjects which produced anti-class I antibodies. FCXM appears useful in estimating posttransplant alloimmune response. Moreover our findings confirm the harmful effects of anti-class I DS-Abs on long-term graft survival.
    Transplant International 02/2000; 13 Suppl 1:S439-43. DOI:10.1007/s001470050378 · 2.60 Impact Factor
Show more