Although there is general agreement that immunohistochemical methods can aid in the pathologic diagnosis of malignant mesothelioma, some studies have produced conflicting results. To obtain comparable and reproducible results, unequivocal malignant mesotheliomas were studied with the biotin-streptavidin-peroxidase complex method in 14 formalin-fixed, paraffin-embedded tissue specimens, 5 ethanol-fixed smear slides and 3 cold acetone-fixed smear slides. The expression of CA-125, epithelial membrane antigen (EMA) and vimentin by malignant epithelial mesothelioma cells was hindered by their poor preservation in formalin fixative. Cytologic specimens fixed in cold acetone were the best type for immunohistochemistry. The majority of malignant epithelial mesothelioma cells in the smear slides fixed in cold acetone were positive for CA-125, EMA, low-molecular-weight cytokeratin and vimentin, but none of them were positive for carcinoembryonic antigen, CA-19-9, epithelial antigen, high-molecular-weight cytokeratin or Leu-M1.
[Show abstract][Hide abstract] ABSTRACT: To document that a polyclonal antiserum to calretinin, a 29-kd calcium-binding protein, consistently decorates normal and tumor mesothelial cells in cytologic preparations.
Thirty-three archival cytologic specimens from eight patients with histologically confirmed malignant mesothelioma and 13 from patients with metastatic serous effusions were destained and then immunostained with anticalretinin antiserum. For investigation of cell suspensions, four pleural fluids were incubated with anticalretinin antiserum. After cytocentrifugation the specimens were stained in accordance with the alkaline phosphatase anti-alkaline phosphatase (APAAP) method. For electron microscopic examination the cell suspensions were then incubated with gold-labeled antirabbit antibody.
The diagnostic sensitivity of this new immunocytochemical approach reached 100% for the eight malignant mesotheliomas investigated. Only 3 of the 13 adenocarcinomas metastatic to the serous membranes included in this study were weakly reactive, accounting for 81% specificity. Binding of anticalretinin antiserum to living mesothelial cells was consistently documented in all four cases investigated.
Calretinin is a very useful marker for positive identification of normal and tumor mesothelial cells in serous effusions.
[Show abstract][Hide abstract] ABSTRACT: Three mesothelioma cell lines (MeET-4, MeET-5, and MeET-6) established from ascitic fluid of F344 rats with spontaneous abdominal
mesothelioma have been maintained through at least 60 passages on the DMEM with 10% FBS. Two of original tumours consisted
of epithelioid cells growing in a papillary patern, while one (original tumour of MeET-5) had sarcomatous areas composed of
spindle-shaped tumour cells. The cell line originating from MeET-5 showed a constantly beiphasic growth pattern during the
repetitive subcloning, while the other two lines retained a monophasic growth pattern. Although the growth pattern was different,
the tumour cells in all three lines were positive for vimentin and keratin and ultrastructurally showed an abundant distribution
of glycogen granules in the cytoplasm and numerous long microvilli on all surface. The modal chromosome number of cell lines
varied from 41 to 71, and abnormal chromosomes were frequently seen. All cell lines established formed colonies on semi-solid
medium and could be successfully transplanted, growing tumour masses in syngeneic rats and thus indicating their malignant
nature. Cell lines grew even on a medium with a low concentration of FBS. The evidence suggests that they may produce growth
factors that enable them to survive unfavourable medium conditions.
Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin 01/1997; 431(4):257-263. DOI:10.1007/s004280050097 · 2.65 Impact Factor
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