Article

Identification of profilin as an IgE-binding component in latex from Hevea brasiliensis: clinical implications.

Faculté de Médecine Alexis Carrel, Lyon, France.
Clinical & Experimental Allergy (impact factor: 5.03). 05/1995; 25(4):332-9. pp.332-9
Source: PubMed

ABSTRACT Considering the high occurrence of profilin as an allergen in many plant species, the assumption was made that profilin might be an allergen in Hevea brasiliensis, a member of the latex producing Euphorbiaceae family. Using IgE-binding inhibition by purified profilins we demonstrated that profilin is an IgE-binding component in the cytosolic fraction of natural latex and, to a lower extent, in the rubber fraction. Thirty-five out of 36 sera containing IgE to ragweed-profilin reacted with profilin from latex, indicating structural homologies between profilins from latex and ragweed. A large percentage (59%) of these sera were found to be positive in CAP latex assay. The preincubation of these sera with purified ragweed profilin greatly inhibited the CAP latex. Because profilin is also present in banana extract, it is likely to be involved in cross-sensitivity to banana and latex. In a group of 19 individuals allergic to latex only two had anti-profilin IgE antibodies. Profilin was barely detectable on glove extract immunoblots, whereas some sera from patients allergic to latex reacted with a 15 kDa allergen which was not profilin. Consequently, IgE antibodies to latex-profilin is a questionable factor for sensitization of occupationally-exposed patients; however, sensitization to profilin should be taken into account when interpreting the results of latex IgE antibody assays.

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Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

15 kDa allergen
 
19 individuals allergic
 
CAP latex
 
CAP latex assay
 
cytosolic fraction
 
Euphorbiaceae family
 
IgE antibodies
 
IgE-binding inhibition
 
latex IgE antibody assays
 
latex-profilin
 
lower extent
 
natural latex
 
patients allergic
 
plant species
 
profilin
 
profilins
 
purified profilins
 
purified ragweed profilin
 
ragweed-profilin
 
structural homologies
 

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