Purification of an ATP-dependent actin-binding protein from a lower eukaryote, Physarum polycephalum.

Department of Pharmacology, Gunma University School of Medicine, Japan.
Biochemical and Biophysical Research Communications (Impact Factor: 2.28). 08/1995; 212(2):347-52. DOI: 10.1006/bbrc.1995.1976
Source: PubMed

ABSTRACT A novel protein with a molecular mass of 55 kDa, as determined by SDS-PAGE, was purified from plasmodia of Physarum polycephalum. The protein bound to actin filaments with a stoichiometry of 0.27 moles per mole of actin with an apparent dissociation constant of 4 x 10(-8) M. In the presence of ATP, the protein dissociated from actin filaments. Adenosine 5-(gamma-thio)triphosphate and adenyl-5'-yl imidodiphosphate also abolished the actin-binding activity of the protein, but GTP did not. Because the cytoplasmic concentration of ATP oscillates in association with the shuttle streaming of the cytoplasm, it is possible that this protein might be involved in the actin-linked regulation of cytoplasmic streaming.

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