Bilateral fetal nigral transplantation into the postcommisural putamen in Parkinson's disease

Department of Surgery, University of South Florida, Tampa, USA.
Annals of Neurology (Impact Factor: 11.91). 09/1995; 38(3):379-88. DOI: 10.1002/ana.410380307
Source: PubMed

ABSTRACT We performed fetal nigral transplantations in 4 Parkinson's disease (PD) patients. Solid grafts were bilaterally implanted into the postcommissural putamen using 3 to 4 donors per side aged 6 1/2 to 9 weeks postconception. Transplant deposits were separated by no more than 5 mm in three dimensions. Cyclosporine was employed for a total of 6 months. Patients were evaluated at baseline and at 1, 3, and 6 months postoperatively. Striatal 18-fluorodopa uptake was assessed by positron emission tomography at baseline and at 6 months postoperatively. The procedure was well tolerated in all patients. One patient had a clinically asymptomatic superficial cortical hemorrhage along the needle tract and a second had transient postoperative confusion and hallucinations. All patients experienced clinically meaningful benefit. Significant improvement (p < 0.05) was detected in total UPDRS score during the "off" state, Schwab-England disability score during the "off" state, percent "off" time, and percent "on" time with dyskinesia. Increased striatal fluorodopa uptake was observed bilaterally in each patient, with mean increases of 53% on the right (p = 0.01) and 33% on the left (p = 0.08). Our study demonstrated clear and consistent improvement in clinical features and striatal fluorodopa uptake following fetal tissue transplantation in patients with advanced PD whose condition was not improved preoperatively by drug manipulation. These preliminary results are encouraging and support further studies to evaluate grafting strategies as a therapy for PD.

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Available from: Cesar V Borlongan, Aug 26, 2015
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    • "Positron emission tomography has also been valuable in monitoring the results of neuronal transplantation trials. While both F-DOPA uptake (Freeman et al, 1995; Kordower et al, 1995; Ma et al, 2010; Figure 2 Positron emission tomography (PET) images depicting cerebral blood flow (CBF), oxygen extraction ratio (OER), and cerebral metabolic rate of oxygen (CMRO 2 ) of a patient after a left hemisphere transient ischemic attack (1ST), 7 hours after a major stroke (2ND), and 4 days after the stroke (3RD). The high oxygen extraction ratio seen within hours after the stroke fell in association with a decline in cortical oxygen metabolism (Wise et al, 1983). "
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    • "Recent studies also suggest that the engraftment of stem cells or progenitors can up-regulate or enhance existing endogenous progenitor populations [12] [13] [14]. Studies have employed neural cell grafts obtained from the fetal ventral mesencephalic (VM) dopaminergic neurons [15] [16] [17] [18] [19] [20]. However, this frequently resulted in significant dyskinesia [21] [22] [23] [24]. "
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    Stem Cell Discovery 10/2011; 1(3):29-43. DOI:10.4236/scd.2011.13004
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    • "When rat VM tissue is hibernated at 4�C for 2–3 days, the survival of dopamine neurons upon subsequent intracerebral grafting is equivalent to what is obtained with fresh tissue (Nikkhah et al., 1995; Sauer and Brundin, 1991). The method has already been used to store tissue used in clinical trials (Freeman et al., 1995; Mendez et al., 2000; Olanow et al., 2003). When the VM tissue is stored for 5–12 days, a gradual drop-off in the survival rate of the grafted dopamine neurons is seen (Nikkhah et al., 1995; Sauer and Brundin, 1991). "
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