Article

Tubulin genes in the algal protist Euglena gracilis.

Department of Biological Sciences (M/C 066), University of Illinois at Chicago 60607-7060.
Journal of Eukaryotic Microbiology (impact factor: 2.66). 41(5):468-77. pp.468-77
Source: PubMed

ABSTRACT Alpha- and beta-tubulin cDNA were selected from a Euglena lambda gt11 expression library, recloned and either sequenced (alpha-tubulin cDNA) or hybridized to Euglena RNA and DNA (alpha- and beta-tubulin cDNA). RNA for hybridization was extracted at 30 minute intervals after flagellar amputation and quantitated for cDNA binding. Unlike previous reports on most other flagellates, no net increase in either alpha- or beta-tubulin RNA could be detected during regeneration--suggesting steady state or constitutive tubulin RNA synthesis. Incubation of the cDNA with genomic DNA after restriction digestion produced patterns of hybridization consistent with the presence of one to two kinds each of the alpha- and beta-tubulin genes. The deduced amino acid sequence of the alpha-tubulin cDNA was more than 90% identical to the alpha-tubulins of Trypanosoma, Chlamydomonas, Naegleria, Tetrahymena and higher plants. The carboxy terminus of the alpha-tubulin cDNA and the previously sequenced beta-tubulin of Euglena showed greatest identity to the carboxy terminus of the tubulins from Trypanosoma brucei. The sequence data for alpha- and beta-tubulins of Euglena provides direct evidence for the similarity of two gene products from euglenas and trypanosomes and adds support to earlier suggestions that these organisms are phylogenetically related.

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    Article: Chloroplast phosphoglycerate kinase from Euglena gracilis: endosymbiotic gene replacement going against the tide.
    Ulrich Nowitzki, Gabriel Gelius-Dietrich, Maike Schwieger, Katrin Henze, William Martin
    [show abstract] [hide abstract]
    ABSTRACT: Two chloroplast phosphoglycerate kinase isoforms from the photosynthetic flagellate Euglena gracilis were purified to homogeneity, partially sequenced, and subsequently cDNAs encoding phosphoglycerate kinase isoenzymes from both the chloroplast and cytosol of E. gracilis were cloned and sequenced. Chloroplast phosphoglycerate kinase, a monomeric enzyme, was encoded as a polyprotein precursor of at least four mature subunits that were separated by conserved tetrapeptides. In a Neighbor-Net analysis of sequence similarity with homologues from numerous prokaryotes and eukaryotes, cytosolic phosphoglycerate kinase of E. gracilis showed the highest similarity to cytosolic and glycosomal homologues from the Kinetoplastida. The chloroplast isoenzyme of E. gracilis did not show a close relationship to sequences from other photosynthetic organisms but was most closely related to cytosolic homologues from animals and fungi.
    European Journal of Biochemistry 11/2004; 271(20):4123-31. · 3.58 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

30 minute intervals
 
90% identical
 
alpha-tubulin cDNA
 
beta-tubulin cDNA
 
beta-tubulin RNA
 
carboxy terminus
 
cDNA
 
cDNA binding
 
constitutive tubulin RNA synthesis
 
direct evidence
 
Euglena lambda gt11 expression library
 
Euglena RNA
 
euglenas
 
genomic DNA
 
higher plants
 
hybridization consistent
 
net increase
 
restriction digestion
 
sequenced beta-tubulin
 
Trypanosoma brucei
 

P J Levasseur